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PMID: 7730257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The fliA gene encoding sigma 28 in Yersinia enterocolitica.

Journal of bacteriology ·Vol. 177 ·No. 9 ·1995-05-00 ·Pages 2299-304

Iriarte M, Stainier I, Mikulskis AV, Cornelis GR

Abstract

Yersinia enterocolitica is an enterobacterium responsible for gastrointestinal syndromes. Its pathogenicity depends on the presence of the 70-kb pYV plasmid, which directs Yop secretion. The Yop secretion machinery, consisting of the YscA-U and LcrD proteins, presents some structural similarity with the flagellum assembly machinery characterized in other bacteria. Flagellum assembly requires sigma 28, an alternative sigma factor. The region upstream of the lcrD gene resembles promoters recognized by sigma 28, suggesting that the similarity between Yop secretion and flagellum assembly could extend to their regulation. The chromosome of Y. enterocolitica also contains pathogenicity determinants such as myfA, which encodes the Myf antigen subunit. The promoter region of myfA also resembles promoters recognized by sigma 28. In an attempt to clarify the role of sigma 28 in the expression of lcrD, myfA, and flagellar genes, we cloned, sequenced, and mutagenized the fliA gene encoding the sigma 28 homolog in Y. enterocolitica. As is the case in other bacteria, fliA was required for motility. However, it was involved neither in fibrilla synthesis nor in Yop secretion. The fliA mutant allowed us to monitor the role of motility in pathogenesis. At least in the mouse model, motility seemed not to be required for Y. enterocolitica pathogenesis.

MeSH Terms
Animals Antigens, Bacterial Antigens, Surface/biosynthesis Bacterial Outer Membrane Proteins/biosynthesis Bacterial Proteins/biosynthesis,genetics Base Sequence Cell Movement/genetics Cloning, Molecular Female Genes, Bacterial/genetics Membrane Proteins/biosynthesis Mice Mice, Inbred BALB C Molecular Sequence Data Peyer's Patches/microbiology Sequence Analysis, DNA Sigma Factor/genetics Spleen/microbiology Transcription, Genetic Virulence/genetics Yersinia enterocolitica/genetics,pathogenicity
Chemicals
Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Proteins FliA protein, Bacteria Membrane Proteins MyfA protein, Yersinia Sigma Factor LcrD protein, Yersinia
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Iriarte M
Microbial Pathogenesis Unit, Université Catholique de Louvain, Brussels, Belgium.
Stainier I
Mikulskis A V
Cornelis G R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.9.2299-2304.1995
Published
1995-05-00
Pages
2299-304
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176884
Subset
IM
Databases
GENBANK
U17393
External Links
PubMed source DOI: 10.1128/jb.177.9.2299-2304.1995 PMC Full Text View journal details
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