Home LiteratureArticle Details
PMID: 7732023 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 9 ·1995-04-25 ·Pages 4011-5

Abu-Elheiga L, Jayakumar A, Baldini A, Chirala SS, Wakil SJ

Abstract

We have cloned and sequenced the cDNA coding for human HepG2 acetyl-CoA carboxylase (ACC; EC 6.4.1.2). The sequence has an open reading frame of 7038 bp that encode 2346 amino acids (M(r), 264,737). The C-terminal 2.6-kb sequence is very different from that recently reported for human ACC (Ha, J., Daniel, S., Kong, I.-S., Park, C.-K., Tae, H.-J. & Kim, K.-H. [1994] Eur. J. Biochem. 219, 297-306). Northern blot analysis revealed that the ACC mRNA is approximately 10 kb in size and that its level varies among the tissues tested. Evidence is presented to show that the human ACC gene is 200-480 kbp in size and maps to chromosome 17q12. We also provide evidence for the presence of another ACC-like gene with similarly sized mRNA but tissue-specific expression different from that of the ACC gene reported herein. That this second ACC-like gene encodes the 280-kDa carboxylase is not ruled out.

MeSH Terms
Acetyl-CoA Carboxylase/biosynthesis,chemistry,genetics Amino Acid Sequence Animals Base Sequence Carcinoma, Hepatocellular Cell Line Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 17 Cloning, Molecular Conserved Sequence DNA, Complementary/chemistry Humans In Situ Hybridization, Fluorescence Isoenzymes/biosynthesis,chemistry,genetics Liver/enzymology Liver Neoplasms Molecular Sequence Data Polymerase Chain Reaction Rats Recombinant Proteins/biosynthesis,chemistry Restriction Mapping Saccharomyces cerevisiae/enzymology Sequence Homology, Amino Acid Tumor Cells, Cultured
Chemicals
DNA, Complementary Isoenzymes Recombinant Proteins Acetyl-CoA Carboxylase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Abu-Elheiga L
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA.
Jayakumar A
Baldini A
Chirala S S
Wakil S J
References (23)
23 references, click to expand
  1. Biotin enzymes.
    Annu Rev Biochem. 1977;46:385-413 PMID: 20039
  2. Isolation and chromosomal mapping of genomic clones encoding the human fatty acid synthase gene.
    Genomics. 1994 Sep 15;23(2):420-4 PMID: 7835891
  3. Carnitine palmitoyltransferase I. The site of inhibition of hepatic fatty acid oxidation by malonyl-CoA.
    J Biol Chem. 1978 Jun 25;253(12):4128-36 PMID: 659409
  4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  5. Fatty acid synthesis and its regulation.
    Annu Rev Biochem. 1983;52:537-79 PMID: 6137188
  6. Kinetics of activation of acetyl-CoA carboxylase by citrate. Relationship to the rate of polymerization of the enzyme.
    J Biol Chem. 1983 Nov 10;258(21):13043-50 PMID: 6138355
  7. The polymerization of acetyl-CoA carboxylase.
    J Biol Chem. 1983 Nov 10;258(21):13051-5 PMID: 6138356
  8. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium.
    In Vitro Cell Dev Biol. 1987 May;23(5):349-54 PMID: 3034851
  9. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  10. Primary structure of chicken liver acetyl-CoA carboxylase deduced from cDNA sequence.
    J Biol Chem. 1988 Feb 25;263(6):2651-7 PMID: 2893793
  11. Regulation of acetyl-coenzyme A carboxylase. I. Purification and properties of two forms of acetyl-coenzyme A carboxylase from rat liver.
    J Biol Chem. 1988 May 5;263(13):6447-53 PMID: 2896193
  12. Structure of the coding sequence and primary amino acid sequence of acetyl-coenzyme A carboxylase.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5784-8 PMID: 2901088
  13. Localization of the gene for acetyl-CoA carboxylase to human chromosome 17.
    Cytogenet Cell Genet. 1988;48(3):190-2 PMID: 2906852
  14. Formation of malonyl coenzyme A in rat heart. Identification and purification of an isozyme of A carboxylase from rat heart.
    J Biol Chem. 1989 Oct 25;264(30):17631-4 PMID: 2572585
  15. Characterization of different forms of rat mammary gland acetyl-coenzyme A carboxylase mRNA: analysis of heterogeneity in the 5' end.
    Gene. 1989 Nov 30;83(2):311-9 PMID: 2573562
  16. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis.
    Annu Rev Genet. 1990;24:37-66 PMID: 2088174
  17. Insulin activation of acetyl-CoA carboxylase accompanied by inhibition of the 5'-AMP-activated protein kinase.
    J Biol Chem. 1992 Feb 15;267(5):2864-7 PMID: 1346611
  18. Cloning of the yeast FAS3 gene and primary structure of yeast acetyl-CoA carboxylase.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4534-8 PMID: 1350093
  19. Acetyl-CoA carboxylase from yeast is an essential enzyme and is regulated by factors that control phospholipid metabolism.
    J Biol Chem. 1993 May 25;268(15):10946-52 PMID: 8098706
  20. Acetyl-CoA carboxylase regulation of fatty acid oxidation in the heart.
    J Biol Chem. 1993 Dec 5;268(34):25836-45 PMID: 7902355
  21. Cloning of human acetyl-CoA carboxylase cDNA.
    Eur J Biochem. 1994 Jan 15;219(1-2):297-306 PMID: 7905825
  22. Unique structural features and differential phosphorylation of the 280-kDa component (isozyme) of rat liver acetyl-CoA carboxylase.
    J Biol Chem. 1994 May 20;269(20):14438-45 PMID: 7910165
  23. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.9.4011
Published
1995-04-25
Pages
4011-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42092
Subset
IM
Grants
NIGMS NIH HHS · GM-19091 · United States
Databases
GENBANK
U19822
External Links
PubMed source DOI: 10.1073/pnas.92.9.4011 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2