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PMID: 775315 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stabilization and size of araC protein.

Molecular & general genetics : MGG ·Vol. 145 ·No. 1 ·1976-04-23 ·Pages 97-100

Wilcox G, Meuris P

Abstract

AraC protein from Escherichia coli has been further stabilized and characterized. pH is a critical variable in conferring stability. araC protein has a sedimentation coefficient of 4.0 +/- 0.2s on standardized 5%-20% glycerol gradients. Its isoelectric point is at a pH of 7.1.

MeSH Terms

Arabinose/metabolism Bacterial Proteins/analysis Escherichia coli/metabolism Isoelectric Point Mutation

Chemicals

Bacterial Proteins Arabinose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wilcox G

Meuris P

References (7)

7 references, click to expand

A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry.
Biochemistry. 1968 Jun;7(6):2207-17 PMID: 5690713
Interaction of the regulatory gene product with the operator site in the L-arabinose operon of Escherichia coli.
J Mol Biol. 1974 Jan 5;85(4):589-602 PMID: 4604623
In vitro activation of the transcription of araBAD operon by araC activator.
Proc Natl Acad Sci U S A. 1974 Mar;71(3):634-8 PMID: 4362626
Regulation of the L-arabinose operon BAD in vitro.
J Biol Chem. 1974 May 10;249(9):2946-52 PMID: 4364034
Synthesis of the arabinose operon regulator protein in a cell-free system.
Mol Gen Genet. 1973 Apr 12;122(2):131-6 PMID: 4349775
Regulation: positive control.
Annu Rev Genet. 1974;8:219-42 PMID: 4374117

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00331563

Published

1976-04-23

Pages

97-100

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00331563

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