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PMID: 7753780 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Transposon tagging of tobacco mosaic virus resistance gene N: its possible role in the TMV-N-mediated signal transduction pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 10 ·1995-05-09 ·Pages 4175-80

Dinesh-Kumar SP, Whitham S, Choi D, Hehl R, Corr C, Baker B

Abstract

Plants can recognize and resist invading pathogens by signaling the induction of rapid defense responses. Often these responses are mediated by single dominant resistance genes (R genes). The products of R genes have been postulated to recognize the pathogen and trigger rapid host defense responses. Here we describe isolation of the classical resistance gene N of tobacco that mediates resistance to the well-characterized pathogen tobacco mosaic virus (TMV). The N gene was isolated by transposon tagging using the maize Activator (Ac) transposon. We confirmed isolation of the N gene by complementation of the TMV-sensitive phenotype with a genomic DNA fragment. Sequence analysis of the N gene shows that it encodes a protein with an amino-terminal domain similar to that of the cytoplasmic domains of the Drosophila Toll protein and the interleukin 1 receptor in mammals, a putative nucleotide-binding site and 14 imperfect leucine-rich repeats. The presence of these functional domains in the predicted N gene product is consistent with the hypothesis that the N resistance gene functions in a signal transduction pathway. Similarities of N to Toll and the interleukin 1 receptor suggest a similar signaling mechanism leading to rapid gene induction and TMV resistance.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Consensus Sequence DNA Transposable Elements Drosophila/genetics Genes, Plant Immunity, Innate/genetics Molecular Sequence Data Mutagenesis, Insertional Phenotype Plants, Toxic Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Signal Transduction Tobacco/genetics,virology Tobacco Mosaic Virus/genetics,pathogenicity Zea mays/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dinesh-Kumar S P
Department of Plant Biology, University of California, Berkeley 94720, USA.
Whitham S
Choi D
Hehl R
Corr C
Baker B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.10.4175
Published
1995-05-09
Pages
4175-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41906
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.10.4175 PMC Full Text View journal details
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