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PMID: 7774594 Published · ppublish English Journal Article

RNA-mediated transposition of the tobacco retrotransposon Tnt1 in Arabidopsis thaliana.

The EMBO journal ·Vol. 14 ·No. 10 ·1995-05-15 ·Pages 2364-73

Lucas H, Feuerbach F, Kunert K, Grandbastien MA, Caboche M

Abstract

The tobacco (Nicotiana tabacum) retrotransposon Tnt1 was introduced into Arabidopsis thaliana. In this heterologous host plant species, Tnt1 undergoes an RNA-mediated transposition and creates a 5 bp duplication at the insertion sites. This is the first report of transposition of a retrotransposon after introduction into a heterologous host species. Tnt1 transposed during in vitro regeneration of transformed A.thaliana, but no transposition event was detected as happening in T2 and T3 generation plants. Newly synthesized copies of Tnt1 can integrate into coding regions of the host DNA. Our results open up the possibility of using Tnt1 as a new tool for insertional mutagenesis and functional analysis of plant genomes, in addition to the strategies of T-DNA and transposon tagging.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Base Sequence Blotting, Northern Blotting, Southern DNA Transposable Elements/genetics Gene Rearrangement/genetics Genetic Markers Genetic Vectors Genome, Plant Meiosis Molecular Sequence Data Plants, Genetically Modified Plants, Toxic Polymerase Chain Reaction RNA, Plant/genetics Recombinant Fusion Proteins/biosynthesis Sequence Analysis, DNA Tissue Distribution Tobacco/genetics Transcription, Genetic Transformation, Genetic
Chemicals
DNA Transposable Elements Genetic Markers RNA, Plant Recombinant Fusion Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lucas H
INRA, Laboratoire de Biologie Cellulaire, Versailles, France.
Feuerbach F
Kunert K
Grandbastien M A
Caboche M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-05-15
Pages
2364-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398345
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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