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PMID: 8389038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterisation of LTR sequences involved in the protoplast specific expression of the tobacco Tnt1 retrotransposon.

Nucleic acids research ·Vol. 21 ·No. 9 ·1993-05-11 ·Pages 2087-93

Casacuberta JM, Grandbastien MA

Abstract

The tobacco Tnt1 retrotransposon is the only plant retrotransposon that has been shown to be transcriptionally active, and its transcription is strongly induced when preparing leaf-derived protoplasts. We have analysed in this paper the LTR sequences important for Tnt1 expression in tobacco protoplasts. We show that LTR sequences upstream of the TATA box are sufficient to confer protoplast-dependent induction to a heterologous promoter. We also show that this region contains two short activator elements, and that one of these sequences, BII, interacts with protoplast-specific nuclear factors.

MeSH Terms
Base Sequence Cloning, Molecular DNA DNA Transposable Elements Gene Expression Regulation Molecular Sequence Data Nuclear Proteins/metabolism Plants, Toxic Promoter Regions, Genetic Protoplasts/metabolism Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid TATA Box Tobacco/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements Nuclear Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Casacuberta J M
Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, France.
Grandbastien M A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.9.2087
Published
1993-05-11
Pages
2087-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309469
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/21.9.2087 PMC Full Text View journal details
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