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PMID: 7777563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 12 ·1995-06-06 ·Pages 5640-4

Desbiez C, David C, Mettouchi A, Laufs J, Gronenborn B

Abstract

The Rep protein of geminiviruses is the sole viral protein required for their DNA replication. The amino acid sequence of Rep protein contains an NTP binding consensus motif (P-loop). Here we show that purified Rep protein of tomato yellow leaf curl virus expressed in Escherichia coli exhibits an ATPase activity in vitro. Amino acid exchanges in the P-loop sequence of Rep causes a substantial decrease or loss of the ATPase activity. In vivo, mutant viruses carrying these Rep mutations do not replicate in plant cells. These results show that ATP binding by the Rep protein of geminiviruses is required for its function in viral DNA replication.

MeSH Terms
Adenosine Triphosphatases/metabolism Amino Acid Sequence DNA Replication DNA, Viral/biosynthesis Geminiviridae/genetics,metabolism Lysine/genetics,metabolism Molecular Sequence Data Nucleotides/metabolism Point Mutation Protein Binding Viral Proteins/chemistry,genetics,metabolism Virus Replication
Chemicals
DNA, Viral Nucleotides Viral Proteins replication protein AL1, Begomovirus Adenosine Triphosphatases Lysine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Desbiez C
Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Yvette, France.
David C
Mettouchi A
Laufs J
Gronenborn B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.12.5640
Published
1995-06-06
Pages
5640-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41752
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.92.12.5640 PMC Full Text View journal details
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