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PMID: 1840909 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Wheat dwarf virus vectors replicate and express foreign genes in cells of monocotyledonous plants.

The Plant cell ·Vol. 3 ·No. 3 ·1991-03-00 ·Pages 247-58

Matzeit V, Schaefer S, Kammann M, Schalk HJ, Schell J, Gronenborn B

Abstract

Wheat dwarf virus (WDV) is a geminivirus that infects monocotyledonous plants. To exploit the potential of WDV as a replicative gene vector, we developed a transient replication and expression system based on the transfection of protoplasts derived from Triticum monococcum suspension culture cells. Cloned genomic copies of various WDV isolates as well as mutants constructed in vitro were introduced into the protoplasts and assayed for their ability to replicate. As a result, regions of the WDV genome necessary or dispensable for the viral DNA replication could be defined. In addition, the gene encoding the viral capsid protein was replaced by three different bacterial marker genes, neomycin phosphotransferase, chloramphenicol acetyltransferase, and beta-galactosidase. The beta-galactosidase gene doubled the size of the WDV genome. The replication of the recombinant WDV genomes and the expression of these genes were monitored in suspension culture cells of T. monococcum. The potential of replicative expression vectors based on the WDV genome is discussed.

MeSH Terms
Base Sequence Cells, Cultured Cloning, Molecular/methods DNA Replication Gene Expression Regulation, Viral Genetic Vectors Molecular Sequence Data Plant Viruses/genetics Protoplasts/microbiology Recombinant Fusion Proteins/biosynthesis Transfection Triticum/genetics,microbiology Virus Replication
Chemicals
Recombinant Fusion Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Matzeit V
Max-Planck-Institut für Züchtungsforschung, Abt. Genetische Grundlagen der Pflanzenzüchtung, Köln, Federal Republic of Germany.
Schaefer S
Kammann M
Schalk H J
Schell J
Gronenborn B
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.3.247
Published
1991-03-00
Pages
247-58
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159996
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.3.3.247 PMC Full Text View journal details
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