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PMID: 7781601 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of the MAP kinase cascade in Xenopus mesoderm induction.

The EMBO journal ·Vol. 14 ·No. 11 ·1995-06-01 ·Pages 2491-8

Gotoh Y, Masuyama N, Suzuki A, Ueno N, Nishida E

Abstract

Mitogen-activated protein kinase (MAPK) is activated by MAPK kinase (MAPKK) in a variety of signaling pathways. This kinase cascade has been shown to function in cell proliferation and differentiation, but its role in early vertebrate development remains to be investigated. During early vertebrate embryogenesis, the induction and patterning of mesoderm are thought to be determined by signals from intercellular factors such as members of the fibroblast growth factor (FGF) family and members of the transforming growth factor-beta family. Here we show that the microinjection of either mRNA encoding a constitutively active mutant of MAPKK or mRNA encoding a constitutively active form of STE11, a MAPKK kinase, leads to the induction of mesoderm in ectodermal explants from Xenopus embryos. Moreover, the expression of MAPK phosphatase-1 (MKP-1, also called CL100) blocks the growth factor-stimulated mesoderm induction. Furthermore, injection of CL100 mRNA into two-cell stage embryos causes severe defects in gastrulation and posterior development. The effects induced by CL100 can be rescued by co-injection of wild-type MAPK mRNA. Thus, the MAPK cascade may play a crucial role in early vertebrate embryogenesis, especially during mesoderm induction.

MeSH Terms
Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases/genetics,metabolism Cell Cycle Proteins DNA Primers/genetics Dual Specificity Phosphatase 1 Immediate-Early Proteins/genetics,metabolism MAP Kinase Kinase Kinases Mesoderm/enzymology Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Mutagenesis, Site-Directed Phosphoprotein Phosphatases Protein Kinases/genetics,metabolism Protein Phosphatase 1 Protein Serine-Threonine Kinases/genetics,metabolism Protein Tyrosine Phosphatases/genetics,metabolism RNA, Messenger/genetics,metabolism,pharmacology Signal Transduction Xenopus/embryology,genetics,metabolism
Chemicals
Cell Cycle Proteins DNA Primers Immediate-Early Proteins RNA, Messenger Protein Kinases Protein Serine-Threonine Kinases Calcium-Calmodulin-Dependent Protein Kinases MAP Kinase Kinase Kinases Mitogen-Activated Protein Kinase Kinases Phosphoprotein Phosphatases Protein Phosphatase 1 Dual Specificity Phosphatase 1 Protein Tyrosine Phosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gotoh Y
Department of Genetics and Molecular Biology, Kyoto University, Japan.
Masuyama N
Suzuki A
Ueno N
Nishida E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-06-01
Pages
2491-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398362
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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