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PMID: 8440264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.

The EMBO journal ·Vol. 12 ·No. 2 ·1993-02-00 ·Pages 787-94

Kosako H, Nishida E, Gotoh Y

Abstract

A Xenopus 45 kDa protein has been identified as an immediate upstream factor sufficient for full activation of MAP kinase, and is shown to be capable of undergoing autophosphorylation on serine, threonine and tyrosine residues. In this study, we show that purified 45 kDa protein can phosphorylate a kinase-negative mutant of Xenopus MAP kinase on tyrosine and threonine residues, suggesting that the 45 kDa protein functions as a MAP kinase kinase to activate MAP kinase. We then report the cloning and sequencing of a full-length cDNA encoding this 45 kDa MAP kinase kinase, and show that it is highly homologous to four protein kinases in fission and budding yeasts: byr1, wis1, PBS2 and STE7. These yeast kinases are therefore suggested to function as a direct upstream activator for a presumed MAP kinase homolog in each signal transduction pathway involved in the regulation of cell cycle progression or cellular responses to extracellular signals. Finally, we report bacterial expression of recombinant MAP kinase kinase that can be phosphorylated and activated by Xenopus egg extracts.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Enzyme Activation Escherichia coli Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Protein Kinases/genetics,metabolism Recombinant Proteins/genetics,metabolism Saccharomyces cerevisiae Schizosaccharomyces Sequence Homology, Amino Acid Xenopus
Chemicals
Recombinant Proteins DNA Protein Kinases Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kosako H
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Nishida E
Gotoh Y
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48 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-02-00
Pages
787-94
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413266
Subset
IM
Databases
GENBANK
D13700
External Links
PubMed source PMC Full Text View journal details
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