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PMID: 7790378 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.

The Journal of cell biology ·Vol. 130 ·No. 1 ·1995-07-00 ·Pages 67-77

Knudsen KA, Soler AP, Johnson KR, Wheelock MJ

Abstract

Cadherins are Ca(2+)-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, transmembrane cadherins such as E-, P-, and N-cadherin self-associate, while intracellularly they interact indirectly with the actin-based cytoskeleton. Several intracellular proteins termed catenins, including alpha-catenin, beta-catenin, and plakoglobin, are tightly associated with these cadherins and serve to link them to the cytoskeleton. Here, we present evidence that in fibroblasts alpha-actinin, but not vinculin, colocalizes extensively with the N-cadherin/catenin complex. This is in contrast to epithelial cells where both cytoskeletal proteins colocalize extensively with E-cadherin and catenins. We further show that alpha-actinin, but not vinculin, coimmunoprecipitates specifically with alpha- and beta-catenin from N- and E-cadherin-expressing cells, but only if alpha-catenin is present. Moreover, we show that alpha-actinin coimmunoprecipitates with the N-cadherin/catenin complex in an actin-independent manner. We therefore propose that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between alpha-actinin and alpha-catenin.

MeSH Terms
Actinin/metabolism Cadherins/metabolism Calcium/physiology Cell Adhesion Cell Line Cytoskeletal Proteins/metabolism Cytoskeleton/ultrastructure Fibroblasts Fluorescent Antibody Technique Humans In Vitro Techniques Macromolecular Substances Precipitin Tests Vinculin/metabolism alpha Catenin
Chemicals
CTNNA1 protein, human Cadherins Cytoskeletal Proteins Macromolecular Substances alpha Catenin Actinin Vinculin Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Knudsen K A
Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096, USA.
Soler A P
Johnson K R
Wheelock M J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.130.1.67
Published
1995-07-00
Pages
67-77
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120515
Subset
IM
Grants
NIGMS NIH HHS · GM 51188 · United States
External Links
PubMed source DOI: 10.1083/jcb.130.1.67 PMC Full Text View journal details
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