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PMID: 7813437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes.

The EMBO journal ·Vol. 13 ·No. 24 ·1994-12-15 ·Pages 5994-6005

Trauth K, Mutschler B, Jenkins NA, Gilbert DJ, Copeland NG, Klempnauer KH

Abstract

C-myb encodes a transcriptional activator that is essential for the development of the hematopoietic system but appears to lack major roles in non-hematopoietic cells. The identification of two conserved myb-related genes, designated A-myb and B-myb, has raised the possibility that these genes are functional equivalents of c-myb in non-hematopoietic cells. Here, we report the isolation and preliminary characterization of the mouse A-myb gene. Mouse A-myb maps to the proximal region of chromosome 1 and encodes a transcriptional activator with properties similar to those of the c-myb and v-myb proteins. During embryo-genesis A-myb is predominantly expressed in several regions of the developing central nervous system (CNS) and the urogenital ridge. Expression in the CNS is confined to the neural tube, the hindbrain, the neural retina and the olfactory epithelium, and coincides with the presence of proliferating immature neuronal precursor cells. In the adult mouse, A-myb is expressed during the early stages of sperm cell differentiation and in B lymphocytes located in germinal centers of the spleen. Taken together, these results suggest a role for A-myb in the proliferation and/or differentiation of neurogenic, spermatogenic and B-lymphoid cells.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/metabolism Base Sequence Chromosome Mapping Crosses, Genetic Gene Expression Regulation Immune System/embryology,growth & development In Situ Hybridization Male Mice Mice, Inbred C57BL Molecular Sequence Data Muridae Nervous System/embryology,metabolism Oncogenes/genetics Proto-Oncogene Proteins/biosynthesis,genetics Proto-Oncogene Proteins c-myb RNA, Messenger/isolation & purification Sequence Analysis, DNA Sequence Homology, Amino Acid Stem Cells/metabolism Testis/embryology,growth & development,metabolism Trans-Activators/biosynthesis,genetics Transcriptional Activation
Chemicals
Mybl1 protein, mouse Proto-Oncogene Proteins Proto-Oncogene Proteins c-myb RNA, Messenger Trans-Activators
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Trauth K
Hans-Spemann-Laboratory, Max-Planck-Institute for Immunobiology, Freiburg, Germany.
Mutschler B
Jenkins N A
Gilbert D J
Copeland N G
Klempnauer K H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-12-15
Pages
5994-6005
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395575
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
Databases
GENBANK
X82327
External Links
PubMed source PMC Full Text View journal details
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