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PMID: 2536148 Published · ppublish English Journal Article

Trans-activation by the c-myb proto-oncogene.

Nucleic acids research ·Vol. 17 ·No. 1 ·1989-01-11 ·Pages 107-17

Nishina Y, Nakagoshi H, Imamoto F, Gonda TJ, Ishii S

Abstract

We present evidence that the mouse c-myb proto-oncogene encodes a transcriptional trans-activator. Trans-activation was assayed by cotransfection into CV1 monkey kidney cells of a c-myb cDNA expression plasmid together with a reporter plasmid carrying the chloramphenicol acetyltransferase (CAT) gene under the control of a test promoter and enhancer. Cotransfection with the c-myb cDNA plasmid caused a 20-fold stimulation of transcription from the promoter of the mouse alpha 2(I) collagen gene linked to tandem repeats of the simian virus 40 (SV40) enhancer element. Using different promoters in combination with varying numbers of repeats of the SV40 enhancer element, it was shown that tandem repeats of the SV40 enhancer mediated the c-myb-induced activation of transcription. These results show that the mouse c-myb gene product either is itself or induces, an activator of transcription that recognizes specific sequences in the SV40 enhancer.

MeSH Terms
Animals Blotting, Southern Cell Line Enhancer Elements, Genetic Gene Expression Regulation Genes Mice Plasmids Promoter Regions, Genetic Proto-Oncogenes Simian virus 40/genetics Transfection
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nishina Y
Laboratory of Molecular Genetics, Tsukuba Life Science Center, Ibaraki, Japan.
Nakagoshi H
Imamoto F
Gonda T J
Ishii S
References (31)
31 references, click to expand
  1. Nucleotide sequence of the transforming gene of avian myeloblastosis virus.
    Science. 1982 Jun 25;216(4553):1421-3 PMID: 6283631
  2. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  3. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  4. Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: the architecture of a transduced oncogene.
    Cell. 1982 Dec;31(2 Pt 1):453-63 PMID: 6297766
  5. Characterization of enhancer elements in the long terminal repeat of Moloney murine sarcoma virus.
    J Virol. 1984 Jan;49(1):183-9 PMID: 6690710
  6. Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.
    Proc Natl Acad Sci U S A. 1984 May;81(10):2980-4 PMID: 6328484
  7. DNA sequence comparison of the regulatory signals at the 5' end of the mouse and chick alpha 2 type I collagen genes.
    J Biol Chem. 1984 Jun 25;259(12):7411-5 PMID: 6203894
  8. Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemia.
    Nature. 1984 Jul 19-25;310(5974):249-51 PMID: 6205276
  9. Regulation of heat shock protein 70 gene expression by c-myc.
    Nature. 1984 Nov 15-21;312(5991):280-2 PMID: 6438521
  10. Regulation of a collagen gene promoter by the product of viral mos oncogene.
    Nature. 1985 Mar 21-27;314(6008):286-9 PMID: 2984572
  11. DNA-binding activity is associated with purified myb proteins from AMV and E26 viruses and is temperature-sensitive for E26 ts mutants.
    Cell. 1985 Apr;40(4):983-90 PMID: 2985272
  12. Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):4920-4 PMID: 2991899
  13. Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements.
    Science. 1985 Nov 22;230(4728):912-6 PMID: 3904002
  14. Nucleotide sequence of cDNA clones of the murine myb proto-oncogene.
    EMBO J. 1985 Aug;4(8):2003-8 PMID: 2998780
  15. Transcriptional activation encoded by the v-fos gene.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3213-7 PMID: 3010284
  16. Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40.
    Nature. 1987 Jan 22-28;325(6102):368-72 PMID: 3027570
  17. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  18. Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities.
    EMBO J. 1987 Apr;6(4):1017-25 PMID: 3036487
  19. The DNA-binding domains of the jun oncoprotein and the yeast GCN4 transcriptional activator protein are functionally homologous.
    Cell. 1987 Sep 11;50(6):841-6 PMID: 3040261
  20. The highly conserved amino-terminal region of the protein encoded by the v-myb oncogene functions as a DNA-binding domain.
    EMBO J. 1987 Sep;6(9):2719-25 PMID: 2824190
  21. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators.
    EMBO J. 1987 Dec 1;6(12):3553-8 PMID: 3428265
  22. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  23. Cell-specific activity of the constituent elements of the simian virus 40 enhancer.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):7881-5 PMID: 2825168
  24. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.
    Nature. 1988 Mar 10;332(6160):166-71 PMID: 3347253
  25. Fos-associated protein p39 is the product of the jun proto-oncogene.
    Science. 1988 May 20;240(4855):1010-6 PMID: 3130660
  26. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
    Science. 1988 Jun 24;240(4860):1759-64 PMID: 3289117
  27. The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of AP-1 responsive genes.
    Cell. 1988 Aug 12;54(4):541-52 PMID: 3135940
  28. fos-associated cellular p39 is related to nuclear transcription factor AP-1.
    Cell. 1988 Aug 12;54(4):553-60 PMID: 3135941
  29. Transcripts from the cellular homologs of retroviral oncogenes: distribution among chicken tissues.
    Mol Cell Biol. 1982 Jun;2(6):617-24 PMID: 14582157
  30. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  31. Differential expression of the amv gene in human hematopoietic cells.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2194-8 PMID: 6954533
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.1.107
Published
1989-01-11
Pages
107-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331538
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.1.107 PMC Full Text View journal details
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