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PMID: 781625 Published · ppublish English Journal Article

A restriction cleavage map of phiX 174 DNA by pulse-chase labelling using E. coli DNA polymerase.

Nucleic acids research ·Vol. 3 ·No. 5 ·1976-05-00 ·Pages 1323-9

Jeppesen PG, Sanders L, Slocombe PM

Abstract

A pulse chase labelling technique using E. coli DNA polymerase I has been used to determine a number of restriction endonuclease cleavage maps of ØX 174 DNA. In addition to verifying the accuracy of the method by confirming some previously published maps, the Hha I and Alu I maps have also been constructed.Images

MeSH Terms
Chromosome Mapping Coliphages/analysis DNA Nucleotidyltransferases/metabolism DNA Restriction Enzymes DNA, Viral/metabolism Escherichia coli/enzymology Haemophilus influenzae/enzymology
Chemicals
DNA, Viral DNA Nucleotidyltransferases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jeppesen P G
Sanders L
Slocombe P M
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.5.1323
Published
1976-05-00
Pages
1323-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342989
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.5.1323 PMC Full Text View journal details
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