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PMID: 4610178 Published · ppublish English Comparative Study Journal Article

Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I.

Journal of virology ·Vol. 14 ·No. 5 ·1974-11-00 ·Pages 1142-51

Hayashi MN, Hayashi M

Abstract

phiX-174 replicative form (RF) DNA was cleaved by restriction enzymes from Haemophilus aphirophilus (Hap) and H. influenzae (H-I strain Osaka) (HinH). Five fragments were produced by Hap and eight by HinH. The positions of all of the Hap fragments and six of the HinH fragments were mapped on the genome by heteroduplex transfection and DNA-DNA hybridization methods. In addition, fragment sizes of phiX-174 RF DNA and S13 RF DNA by Hap and HinH were compared. Considerable differences of the size of the fragments produced from these closely related phage genomes were observed.

MeSH Terms
Chromosome Mapping Coliphages/analysis Crosses, Genetic DNA Replication DNA Viruses DNA, Circular/analysis,metabolism DNA, Viral/analysis,metabolism Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Escherichia coli Genes Haemophilus/enzymology Haemophilus influenzae/enzymology Mutation Nucleic Acid Denaturation Nucleic Acid Hybridization Phosphorus Radioisotopes Recombination, Genetic Spheroplasts Thymidine Transformation, Genetic Tritium
Chemicals
DNA, Circular DNA, Viral Phosphorus Radioisotopes Tritium Endonucleases Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hayashi M N
Hayashi M
References (20)
20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.14.5.1142-1151.1974
Published
1974-11-00
Pages
1142-51
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355631
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.14.5.1142-1151.1974 PMC Full Text View journal details
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