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PMID: 7828821 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The effect of an intronic polymorphism on alcohol dehydrogenase expression in Drosophila melanogaster.

Genetics ·Vol. 138 ·No. 2 ·1994-10-00 ·Pages 379-85

Laurie CC, Stam LF

Abstract

Several lines of evidence indicate that natural selection controls the frequencies of an allozyme polymorphism at the alcohol dehydrogenase (Adh) locus in Drosophila melanogaster. However, because of associations among sequence polymorphisms in the Adh region, it is not clear whether selection acts directly (or solely) on the allozymic site. This problem has been approached by using in vitro mutagenesis to distinguish among the effects on Adh expression of individual polymorphisms. This study shows that a polymorphism within the first Adh intron (delta 1) has a significant effect on the level of ADH protein. Like the allozyme, delta 1 shows a geographic cline in frequency, indicating that it may also be a target of natural selection. These results suggest that multisite selection models may be required to understand the evolutionary dynamics of individual loci.

Related Genes
Adh
MeSH Terms
Alcohol Dehydrogenase/biosynthesis,genetics Animals Animals, Genetically Modified Drosophila melanogaster/enzymology,genetics Gene Expression Regulation, Enzymologic Introns Polymerase Chain Reaction/methods Polymorphism, Genetic RNA, Messenger/biosynthesis Recombination, Genetic Restriction Mapping Transcription, Genetic
Chemicals
RNA, Messenger Alcohol Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laurie C C
Department of Zoology, Duke University, Durham, North Carolina 27708.
Stam L F
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/138.2.379
Published
1994-10-00
Pages
379-85
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206156
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/138.2.379 PMC Full Text View journal details
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