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PMID: 783123 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Bacteriophage-resistant mutants of Salmonella typhimurium deficient in two major outer membrane proteins.

Journal of bacteriology ·Vol. 127 ·No. 2 ·1976-08-00 ·Pages 941-55

Nurminen M, Lounatmaa K, Sarvas M, Mäkelä PH, Nakae T

Abstract

Mutants resistant to bacteriophages (P221 and PH105 or PH51) were isolated from a rfa strain of Salmonella typhimurium. They were found deficient in separate 33,000- to 36,000-dalton band proteins (major band proteins). Double mutants derived from both types of mutants were deficient in both of the bands. The growth behavior of all the mutants was normal. The outer membrane of the mutants appeared to be more wrinkled than normal and formed vesicles in many of the mutants. In freeze-fractured cells, changes were seen in the outer membrane (particleless patches in the concave fracture face, the particles themselves being smaller than normal). These changes were more marked in the double mutants.

MeSH Terms
Adsorption Bacterial Proteins/biosynthesis Binding Sites Cell Wall/metabolism,ultrastructure Lysogeny Molecular Weight Mutation Salmonella Phages/growth & development Salmonella typhimurium/metabolism,ultrastructure Virus Replication
Chemicals
Bacterial Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nurminen M
Lounatmaa K
Sarvas M
Mäkelä P H
Nakae T
References (27)
27 references, click to expand
  1. Covalent lipoprotein from the outer membrane of Escherichia coli.
    Biochim Biophys Acta. 1975 Oct 31;415(3):335-77 PMID: 52377
  2. Outer membrane of Salmonella. Isolation of protein complex that produces transmembrane channels.
    J Biol Chem. 1976 Apr 10;251(7):2176-8 PMID: 773934
  3. Mutants of Escherichia coli K12 lacking all 'major' proteins of the outer cell envelope membrane.
    FEBS Lett. 1975 Jul 15;55(1):161-4 PMID: 806476
  4. The major proteins of the Excherichia coli outer cell envelope membrane. Preparative isolation of all major membrane proteins.
    Eur J Biochem. 1975 Nov 1;59(1):207-13 PMID: 812694
  5. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A.
    J Bacteriol. 1975 Jul;123(1):102-17 PMID: 1095546
  6. Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants.
    J Bacteriol. 1975 Nov;124(2):942-58 PMID: 1102538
  7. The major proteins of the Escherichia coli outer cell envelope membrane. Characterization of proteins II* and III, comparison of all proteins.
    Eur J Biochem. 1975 Nov 1;59(1):215-21 PMID: 1107024
  8. Alterations in envelope structure of heptose-deficient mutants of Escherichia coli as revealed by freeze-etching.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5145-9 PMID: 1108014
  9. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
    J Biol Chem. 1974 Jan 25;249(2):634-44 PMID: 4129205
  10. Outer membrane proteins of Escherichia coli. 3. Evidence that the major protein of Escherichia coli O111 outer membrane consists of four distinct polypeptide species.
    J Bacteriol. 1974 May;118(2):442-53 PMID: 4208134
  11. Permeability of lipopolysaccharide-deficient (rough) mutants of Salmonella typhimurium to antibiotics, lysozyme, and other agents.
    Can J Microbiol. 1974 Aug;20(8):1135-45 PMID: 4213795
  12. Purification and properties of the colicin E3 receptor of Escherichia coli.
    J Biol Chem. 1973 Mar 10;248(5):1797-806 PMID: 4348549
  13. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  14. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping.
    J Gen Microbiol. 1972 May;70(3):527-54 PMID: 4556257
  15. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.
    J Bacteriol. 1974 Feb;117(2):406-16 PMID: 4590466
  16. Outer membrane proteins of Escherichia coli. IV. Differences in outer membrane proteins due to strain and cultural differences.
    J Bacteriol. 1974 May;118(2):454-64 PMID: 4597444
  17. Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage.
    J Bacteriol. 1974 Sep;119(3):726-35 PMID: 4604263
  18. The origin of bacteriophage P221.
    Virology. 1967 Nov;33(3):545-7 PMID: 4863176
  19. [Growth and lipopolysaccharide (O-antigen) levels of salmonella in cultures of agar media].
    Zentralbl Bakteriol Orig. 1966 Jun;200(2):241-59 PMID: 4869922
  20. ES18, a general transducing phage for smooth and nonsmooth Salmonella typhimurium.
    Virology. 1970 Nov;42(3):621-32 PMID: 4921684
  21. Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli.
    J Bacteriol. 1971 Oct;108(1):553-63 PMID: 5001205
  22. Fracture faces of frozen membranes.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1048-56 PMID: 5334198
  23. [Investigations on the classification of salmonella R forms. 3. Classification of S. minnesota mutants by chemical agents].
    Zentralbl Bakteriol Orig. 1969 Dec;212(1):88-96 PMID: 5386255
  24. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  25. A new gene cluster rfe concerned with the biosynthesis of Salmonella lipopolysaccharide.
    J Gen Microbiol. 1970 Jan;60(1):91-106 PMID: 5488469
  26. Genomic masking and recombination between serologically unrelated phages P22 and P221.
    Virology. 1961 Aug;14:430-9 PMID: 13787142
  27. Temperate Bacteriophage Which Causes the Production of a New Major Outer Membrane Protein by Escherichia coli.
    J Virol. 1975 May;15(5):1121-30 PMID: 16789148
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.127.2.941-955.1976
Published
1976-08-00
Pages
941-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233004
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.127.2.941-955.1976 PMC Full Text View journal details
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