Abstract
The mod (chlD) locus at 17 min on the Escherichia coli chromosome encodes a high-affinity molybdate uptake system. To further investigate the structure and regulation of these genes, the DNA region upstream of the previously identified modBC (chlJD) genes was cloned and sequenced. A single open reading frame, designated modA, was identified and appears to encode a periplasmic binding protein for the molybdate uptake system. To determine how the mod genes are regulated in response to molybdate, nitrate, and oxygen, we constructed a series of mod-lacZ operon fusions to the upstream region and introduced them in single copy onto the E. coli chromosome. Whereas molybdate limitation resulted in elevated mod-lacZ expression, neither oxygen nor nitrate had any significant effect on gene expression. A regulatory motif, CATAA, located at the modA promoter was identified and shown to be required for molybdate-dependent control of the modABCD operon. Mutations within this sequence resulted in nearly complete derepression of gene expression and suggest that transcription of the operon is mediated by a molybdenum-responsive regulatory protein.
MeSH Terms
Alleles
Bacterial Proteins/genetics
Base Sequence
Biological Transport
Coenzymes
DNA-Binding Proteins
Escherichia coli/drug effects,genetics
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Membrane Proteins/genetics
Metalloproteins/metabolism
Molecular Sequence Data
Molybdenum/metabolism
Molybdenum Cofactors
Mutation
Nitrates/pharmacology
Operon/genetics
Oxygen/pharmacology
Phosphoproteins/genetics
Promoter Regions, Genetic/genetics
Protein Kinases
Pteridines/metabolism
Recombinant Fusion Proteins/biosynthesis
Repressor Proteins/genetics
Chemicals
Bacterial Proteins
Coenzymes
DNA-Binding Proteins
Escherichia coli Proteins
Membrane Proteins
Metalloproteins
Molybdenum Cofactors
Nitrates
Phosphoproteins
Pteridines
Recombinant Fusion Proteins
Repressor Proteins
ferric uptake regulating proteins, bacterial
narQ protein, E coli
NarL protein, E coli
molybdate
Molybdenum
molybdenum cofactor
Protein Kinases
narX protein, E coli
Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rech S
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
Deppenmeier U
Gunsalus R P
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