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PMID: 7860604 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli.

Journal of bacteriology ·Vol. 177 ·No. 4 ·1995-02-00 ·Pages 938-47

Xu J, Johnson RC

Abstract

Fis is a nucleoid-associated protein in Escherichia coli that has been shown to regulate recombination, replication, and transcription reactions. It is expressed in a transient manner under batch culturing conditions such that high levels are present during early exponential phase and low levels are present during late exponential phase and stationary phase. We have screened a random collection of transposon-induced lac fusions for those which give decreased expression in the presence of Fis. Thirteen different Fis-repressed genes were identified, including glnQ (glutamine high-affinity transport), mglA (methyl-galactoside transport), xylF (D-xylose-binding protein), sdhA (succinate dehydrogenase flavoprotein subunit), and a newly identified aldehyde dehydrogenase, aldB. The LacZ expression patterns revealed that many of the fusions were maximally expressed at different stages of growth, including early log phase, mid- to late log phase, and stationary phase. The expression of some of the late-exponential- and stationary-phase genes was dependent on the RpoS sigma factor, whereas that of others was affected negatively by RpoS. We conclude that Fis negatively regulates a diverse set of genes and that RpoS can function to both activate and inhibit the expression of specific genes.

Related Genes
aldB fis glnQ mglA rpoS sdhA xylF
MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,metabolism Base Sequence Carboxy-Lyases/biosynthesis Carrier Proteins/biosynthesis,metabolism Escherichia coli/genetics,growth & development Escherichia coli Proteins Factor For Inversion Stimulation Protein Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Integration Host Factors Molecular Sequence Data Mutagenesis, Insertional Recombinant Fusion Proteins/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Sigma Factor/metabolism Succinate Dehydrogenase/biosynthesis Symporters
Chemicals
Bacterial Proteins Carrier Proteins Escherichia coli Proteins Factor For Inversion Stimulation Protein Integration Host Factors Recombinant Fusion Proteins Sigma Factor Symporters integration host factor, E coli sigma factor KatF protein, Bacteria xylE protein, E coli MglA protein, Myxococcus xanthus Succinate Dehydrogenase Carboxy-Lyases acetolactate decarboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xu J
Department of Biological Chemistry, UCLA School of Medicine 90024.
Johnson R C
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45 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.4.938-947.1995
Published
1995-02-00
Pages
938-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176687
Subset
IM
Grants
NIGMS NIH HHS · GM38509 · United States
External Links
PubMed source DOI: 10.1128/jb.177.4.938-947.1995 PMC Full Text View journal details
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