Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences.

Journal of bacteriology ·Vol. 177 ·No. 5 ·1995-03-00 ·Pages 1414-7

Ueda T, Suga Y, Yahiro N, Matsuguchi T

Abstract

To demonstrate the extent of phylogenetic diversity of diazotrophic bacteria associated with rice roots, we characterized phylogenetically 23 nifH gene sequences obtained by PCR amplification of mixed organism DNA extracted directly from rice roots without culturing the organisms. The analyses document the presence of eight novel NifH types, which appear to be a variety of significant components of the diazotrophic community, dominated mainly by proteobacteria.

MeSH Terms

Amino Acid Sequence Bacteria/enzymology,genetics Biological Evolution DNA, Bacterial/genetics Gene Library Genes, Bacterial Genetic Variation Molecular Sequence Data Nitrogen Fixation/genetics Nitrogenase/classification,genetics Oryza/microbiology Oxidoreductases Phylogeny Plant Roots/microbiology Polymerase Chain Reaction Sequence Analysis, DNA

Chemicals

DNA, Bacterial Oxidoreductases Nitrogenase nitrogenase reductase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ueda T

Department of Agricultural Chemistry, Kyushu University, Fukuoka, Japan.

Suga Y

Yahiro N

Matsuguchi T

References (5)

5 references, click to expand

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Mol Biol Evol. 1987 Jul;4(4):406-25 PMID: 3447015
Compilation of small ribosomal subunit RNA structures.
Nucleic Acids Res. 1993 Jul 1;21(13):3025-49 PMID: 8332525
Rapid method for direct extraction of DNA from soil and sediments.
Appl Environ Microbiol. 1991 Apr;57(4):1070-4 PMID: 1647749
Phylogeny of nitrogenase sequences in Frankia and other nitrogen-fixing microorganisms.
J Mol Evol. 1989 Nov;29(5):436-47 PMID: 2515293

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.5.1414-1417.1995

Published

1995-03-00

Pages

1414-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC176754

Subset

Databases

GENBANK

D26284, D26285, D26286, D26287, D26288, D26289, D26290, D26291, D26292, D26293, D26294, D26295, D26296, D26297, D26298, D26299, D26300, D26301, D26302, D26303, D26304, D26305, D26306

External Links

PubMed source DOI: 10.1128/jb.177.5.1414-1417.1995 PMC Full Text View journal details

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Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences.

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