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PMID: 2515293 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Phylogeny of nitrogenase sequences in Frankia and other nitrogen-fixing microorganisms.

Journal of molecular evolution ·Vol. 29 ·No. 5 ·1989-11-00 ·Pages 436-47

Normand P, Bousquet J

Abstract

The complete nucleotide sequence of a nitrogenase (nifH) gene was determined from a second strain (HRN18a) of Frankia, an aerobic soil bacterium. The open reading frame is 870 bp long and encodes a polypeptide of 290 amino acids. The amino acid and nucleotide sequences were compared with 21 other published sequences. The two Frankia strains were 96% similar at the amino acid level and 93% similar at the nucleotide level. A number of methods were used to infer phylogenies of these nitrogen fixers, based on nifH amino acid and nucleotide sequences. The results obtained do not agree completely with other phylogenies for these bacteria and thus make probable occurrences of lateral transfer of the nif genes. The time of divergence of the two Frankia strains could be estimated at about 100 million years. The vanadium-dependent (Type 2) nitrogenase present in Azotobacter spp. appears to be a recent derivation from the conventional molybdenum-dependent (Type 1) enzyme, whereas the iron-dependent (Type 3) alternative nitrogenase would have a much older origin.

MeSH Terms
Actinomyces/genetics Amino Acid Sequence Base Composition Base Sequence Molecular Sequence Data Nitrogen Fixation Nitrogenase/genetics Phylogeny
Chemicals
Nitrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Normand P
Centre de Recherche en Biologie Forestière, Université Laval, Québec, Canada.
Bousquet J
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02602914
Published
1989-11-00
Pages
436-47
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02602914 View journal details
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