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PMID: 787982 Published · ppublish English Journal Article

Circular DNA of a yeast episome with two inverted repeats: structural analysis by a restriction enzyme and electron microscopy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3030-4

Guerineau M, Grandchamp C, Slonimski PP

Abstract

Small circular DNA molecules from genetically characterized clones of Saccharomyces cerevisiae have been studied by restriction endonuclease analysis and electron microscopy. The circular monomers (6000 bases) are shown to contain two inverted repeats of the same sequence (600 bases) situated opposite each other along the perimeter. Four endonuclease EcoRI fragments are obtained in 1:1:1:1 stoichiometry, and their sum gives a length of about 12,000 bases. The two large fragments and the two small ones differ from each other by 200 bases. We propose a model for the structure of the monomer molecule. Two classes of monomers can be generated by intramolecular recombinations within inverted repeats; they differ by the relative orientation of nonrepeated segments. The structure of dimers as predicted by the model is verified by self-renaturation of single-stranded circles. Inverted repeats in circular molecules may be related to the insertion release faculty of II episome in the chromosomes.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Circular/analysis Extrachromosomal Inheritance Microscopy, Electron Nucleic Acid Denaturation Nucleic Acid Renaturation Plasmids Saccharomyces cerevisiae
Chemicals
DNA, Circular DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guerineau M
Grandchamp C
Slonimski P P
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.9.3030
Published
1976-09-00
Pages
3030-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430917
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.9.3030 PMC Full Text View journal details
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