Home LiteratureArticle Details
PMID: 7894014 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The isocitrate lyase gene of cucumber: isolation, characterisation and expression in cotyledons following seed germination.

Plant molecular biology ·Vol. 27 ·No. 3 ·1995-02-00 ·Pages 487-97

Reynolds SJ, Smith SM

Abstract

The cucumber (Cucumis sativus L.) genome contains only a single gene encoding the glyoxylate cycle enzyme isocitrate lyase (ICL). The cucumber icl gene has been isolated and sequenced, revealing only two small introns. The predicted amino acid sequence is more than 85% identical to ICL from other higher plants, and contains the C-terminal tripeptide Ser-Arg-Met which resembles a peroxisomal targeting sequence. The icl gene is coordinately expressed with the malate synthase (ms) gene after seed germination in both the light and the dark, suggesting that these genes may contain similar DNA elements regulating transcription. The start of transcription of the icl gene was determined and the DNA sequences upstream compared with the region of the ms gene promoter known to regulate transcription. This comparison revealed a highly conserved DNA sequence at similar positions in each gene.

Related Genes
icl
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cotyledon/metabolism Cucumis sativus/enzymology,genetics Gene Expression Regulation, Plant Genes, Plant/genetics Introns/genetics Isocitrate Lyase/chemistry,genetics Malate Synthase/genetics Molecular Sequence Data Promoter Regions, Genetic/genetics RNA, Messenger/biosynthesis RNA, Plant/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic/genetics
Chemicals
RNA, Messenger RNA, Plant Malate Synthase Isocitrate Lyase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reynolds S J
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Smith S M
References (43)
43 references, click to expand
  1. Molecular cloning of cucumber phosphoenolpyruvate carboxykinase and developmental regulation of gene expression.
    Plant Mol Biol. 1994 Oct;26(1):423-34 PMID: 7948888
  2. Regulation of glyoxysomal enzymes during germination of cucumber. Temporal changes in translatable mRNAs for isocitrate lyase and malate synthase.
    Eur J Biochem. 1980 Dec;112(3):469-77 PMID: 6161814
  3. Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli.
    J Bacteriol. 1988 Oct;170(10):4528-36 PMID: 3049537
  4. Structure and regulation of the isocitrate lyase gene ICL1 from the yeast Saccharomyces cerevisiae.
    Curr Genet. 1993 May-Jun;23(5-6):375-81 PMID: 8319292
  5. Synthesis of C4-dicarboxylic acids from acetate by a glyoxylate bypass of the tricarboxylic acid cycle.
    Biochim Biophys Acta. 1957 Jun;24(3):651-3 PMID: 13436500
  6. Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis.
    Nucleic Acids Res. 1987 Dec 10;15(23):9627-40 PMID: 3697078
  7. A rapid method for isolating high quality plasmid DNA suitable for DNA sequencing.
    Nucleic Acids Res. 1990 Dec 25;18(24):7463-4 PMID: 2259645
  8. Nucleic acid (cDNA) and amino acid sequences of isocitrate lyase from castor bean.
    Plant Mol Biol. 1987 Nov;8(6):471-5 PMID: 24301309
  9. Identification of a cysteine residue at the active site of Escherichia coli isocitrate lyase.
    Biochem J. 1989 Jul 15;261(2):431-5 PMID: 2673221
  10. Identification of peroxisomal targeting signals located at the carboxy terminus of four peroxisomal proteins.
    J Cell Biol. 1988 Sep;107(3):897-905 PMID: 2901422
  11. Induction of Malate Synthase Gene Expression in Senescent and Detached Organs of Cucumber.
    Plant Cell. 1992 Mar;4(3):349-357 PMID: 12297649
  12. Coordinate expression of transcriptionally regulated isocitrate lyase and malate synthase genes in Brassica napus L.
    Plant Cell. 1989 Mar;1(3):293-300 PMID: 2535504
  13. Peroxisomal enzyme activities in attached senescing leaves.
    Planta. 1991 Apr;184(1):151-3 PMID: 24193942
  14. The carboxyl terminus of isocitrate lyase is not essential for import into glyoxysomes in an in vitro system.
    J Biol Chem. 1993 Apr 5;268(10):7315-22 PMID: 8463266
  15. Localization of glyoxylate-cycle marker enzymes in peroxisomes of senescent leaves and green cotyledons.
    Planta. 1990 Feb;180(3):435-9 PMID: 24202025
  16. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  17. Cloning of the isocitrate lyase gene (ICL1) from Yarrowia lipolytica and characterization of the deduced protein.
    Mol Gen Genet. 1993 Nov;241(3-4):422-30 PMID: 8246896
  18. Vanadate-dependent photomodification of serine 319 and 321 in the active site of isocitrate lyase from Escherichia coli.
    J Biol Chem. 1992 Jan 5;267(1):91-5 PMID: 1730628
  19. Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3).
    Curr Genet. 1992 Jan;21(1):43-7 PMID: 1531185
  20. Two classes of isocitrate lyase genes are expressed during late embryogeny and postgermination in Brassica napus L.
    Mol Gen Genet. 1993 Apr;238(1-2):177-84 PMID: 8479425
  21. The contribution of AAUAAA and the upstream element UUUGUA to the efficiency of mRNA 3'-end formation in plants.
    EMBO J. 1994 May 1;13(9):2200-10 PMID: 8187773
  22. Development and regulation of three glyoxysomal enzymes during cotton seed maturation and growth.
    Plant Mol Biol. 1990 Feb;14(2):137-46 PMID: 1714313
  23. Glyoxysomal Malate Synthase of Cucumber: Molecular Cloning of a cDNA and Regulation of Enzyme Synthesis during Germination.
    Plant Physiol. 1986 Jul;81(3):762-7 PMID: 16664899
  24. Carbon Catabolite Repression Regulates Glyoxylate Cycle Gene Expression in Cucumber.
    Plant Cell. 1994 May;6(5):761-772 PMID: 12244257
  25. Metabolic production of sucrose from fat.
    Nature. 1961 Jul 29;191:433-6 PMID: 13688650
  26. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  27. Thiolase mRNA translated in vitro yields a peptide with a putative N-terminal presequence.
    Plant Mol Biol. 1993 Apr;22(1):59-66 PMID: 8098965
  28. Targeting of glyoxysomal proteins to peroxisomes in leaves and roots of a higher plant.
    Plant Cell. 1993 Aug;5(8):941-52 PMID: 8400872
  29. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  30. Regulation of isocitrate lyase gene expression in sunflower.
    Plant Physiol. 1988 Feb;86(2):527-32 PMID: 16665941
  31. Alkylation of isocitrate lyase from Escherichia coli by 3-bromopyruvate.
    Arch Biochem Biophys. 1990 May 1;278(2):373-80 PMID: 2183722
  32. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  33. Apparent induction of key enzymes of the glyoxylic acid cycle in senescent barley leaves.
    Planta. 1988 Dec;176(4):548-50 PMID: 24220952
  34. An inspection of the domain between putative TATA box and translation start site in 79 plant genes.
    Nucleic Acids Res. 1987 Aug 25;15(16):6643-53 PMID: 3628002
  35. Isocitrate lyase and malate synthase genes from Brassica napus L. are active in pollen.
    Plant Physiol. 1994 Mar;104(3):857-64 PMID: 8165259
  36. Characterization of a cDNA clone encoding the complete amino acid sequence of cotton isocitrate lyase.
    Biochim Biophys Acta. 1990 Jun 21;1049(2):223-6 PMID: 2194576
  37. Developmental regulation of expression of the malate synthase gene in transgenic plants.
    Plant Mol Biol. 1990 Oct;15(4):539-49 PMID: 2102373
  38. Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber.
    Plant Mol Biol. 1994 Dec;26(6):1833-41 PMID: 7858221
  39. In vivo import of firefly luciferase into the glycosomes of Trypanosoma brucei and mutational analysis of the C-terminal targeting signal.
    Mol Biol Cell. 1992 Jul;3(7):749-59 PMID: 1515676
  40. Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans.
    J Gen Microbiol. 1976 Feb;92(2):263-82 PMID: 3622
  41. Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage.
    Cell. 1991 Apr 5;65(1):115-23 PMID: 2013092
  42. Peroxisomal isocitrate lyase of the n-alkane-assimilating yeast Candida tropicalis: gene analysis and characterization.
    J Biochem. 1990 Feb;107(2):262-6 PMID: 2361956
  43. The malate synthase gene of cucumber.
    Plant Mol Biol. 1989 Dec;13(6):673-84 PMID: 2491683
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019316
Published
1995-02-00
Pages
487-97
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
Z35499
External Links
PubMed source DOI: 10.1007/BF00019316 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2