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PMID: 7913928 Published · ppublish English Comparative Study Journal Article

Analysis of the distribution and molecular heterogeneity of the ospD gene among the Lyme disease spirochetes: evidence for lateral gene exchange.

Journal of bacteriology ·Vol. 176 ·No. 15 ·1994-08-00 ·Pages 4572-82

Marconi RT, Samuels DS, Landry RK, Garon CF

Abstract

Analysis of the ospD gene has revealed that this gene is not universal among Lyme disease spirochete isolates. The gene was found to be carried by 90, 50, and 24% of the Borrelia garinii, B. afzelii, and B. burgdorferi isolates tested. Size variability in the ospD-encoding plasmid was also observed. Sequence analysis has demonstrated the presence of various numbers of a 17-bp repeated sequence in the upstream control (promoter) region of the gene. In addition, a region within the coding sequence where various insertions, deletions, and direct repeats occur was identified. ospD gene sequences from 31 different isolates were determined and utilized in pairwise sequence comparisons and construction of a gene tree. These analyses suggest that the ospD gene was the target of several recombinational events and that the gene was recently acquired by Lyme disease spirochetes and laterally transferred between species.

Related Genes
ospD
MeSH Terms
Amino Acid Sequence Animals Bacterial Outer Membrane Proteins/genetics Base Sequence Borrelia/classification,genetics Borrelia burgdorferi Group/classification,genetics Cloning, Molecular Genes, Bacterial/genetics Genetic Variation Humans Lyme Disease/microbiology Molecular Sequence Data Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
Bacterial Outer Membrane Proteins outer surface protein D, Borrelia burgdorferi
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marconi R T
Laboratory of Vectors and Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
Samuels D S
Landry R K
Garon C F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.15.4572-4582.1994
Published
1994-08-00
Pages
4572-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196277
Subset
IM
Databases
GENBANK
U05304, U05305, U05306, U05322, U05323, U05324, U05325, U05326, U05327, U05328, U05329, U05337
External Links
PubMed source DOI: 10.1128/jb.176.15.4572-4582.1994 PMC Full Text View journal details
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