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PMID: 7927773 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of hifD and hifE in the pilus gene cluster of Haemophilus influenzae type b strain Eagan.

Infection and immunity ·Vol. 62 ·No. 11 ·1994-11-00 ·Pages 4922-8

McCrea KW, Watson WJ, Gilsdorf JR, Marrs CF

Abstract

Haemophilus influenzae produces surface structures called pili that promote adherence to human cells. Three genes encoding the major pilus structural component (pilin), chaperone, and usher proteins (designated hifA, -B, and -C, respectively) have been identified previously. In this study, transposon mutagenesis and DNA sequence analysis identified two open reading frames (ORFs) downstream of, and in the same orientation as, hifC. These genes have been designated hifD and hifE. Both genes have predicted C-terminal amino acid homology to HifA, and mutations in either gene resulted in the loss of morphologic and functional pili, indicating that hifD and hifE encode pilus structural components and are required for pilus expression. Another ORF, identified immediately downstream of hifE, has a predicted amino acid sequence that is 70% identical to an aminopeptidase of Escherichia coli called PepN, and a mutation within this ORF did not alter pilus expression. These data indicate that the pepN homolog is not required for pilus biogenesis and that one end of the pilus gene cluster has been defined.

Related Genes
hifB hifC hifD hifE infA pepN
MeSH Terms
Adhesins, Bacterial/genetics Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/genetics Base Sequence DNA, Bacterial/genetics Fimbriae Proteins Fimbriae, Bacterial/ultrastructure Gene Expression Regulation, Bacterial Genes, Bacterial Haemophilus influenzae/genetics,ultrastructure Molecular Chaperones/genetics Molecular Sequence Data Multigene Family Operon Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Alignment Sequence Deletion Sequence Homology, Amino Acid
Chemicals
Adhesins, Bacterial Bacterial Proteins DNA, Bacterial HifD protein, Haemophilus influenzae HifE protein, Haemophilus influenzae Molecular Chaperones Fimbriae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McCrea K W
Department of Epidemiology, University of Michigan, Ann Arbor 48109.
Watson W J
Gilsdorf J R
Marrs C F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.11.4922-4928.1994
Published
1994-11-00
Pages
4922-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC303208
Subset
IM
Grants
NIAID NIH HHS · R01-AI25630 · United States
Databases
GENBANK
U13254
External Links
PubMed source DOI: 10.1128/iai.62.11.4922-4928.1994 PMC Full Text View journal details
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