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PMID: 7929767 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease.

Journal of clinical microbiology ·Vol. 32 ·No. 7 ·1994-07-00 ·Pages 1733-8

Padula SJ, Dias F, Sampieri A, Craven RB, Ryan RW

Abstract

Infection with Borrelia burgdorferi, the etiologic agent of Lyme disease, is associated with an early and dominant humoral response to the spirochete's 23-kDa outer surface protein C (OspC). We have cloned and expressed OspC as a fusion protein in Escherichia coli and have shown that patient serum samples react with it in an enzyme-linked immunosorbent assay (ELISA) (S. J. Padula, A. Sampieri, F. Dias, A. Szczepanski, and R. W. Ryan, Infect. Immun. 61:5097-5105, 1993). Now we have compared the detection of B. burgdorferi-specific immunoglobulin M antibodies in 74 individuals with culture-positive erythema migrans by a whole-cell ELISA, immunoblot, and the recombinant OspC (rOspC) ELISA. Seventy-six negative controls were also studied. With all of the tests, there was a statistically significant association between the duration of disease and the frequency of a positive result. With the rOspC ELISA, the predictive value of a positive test was 100% and the predictive value of a negative test was 74%. Similar results were obtained with the whole-cell ELISA and with the immunoblot using as the source of test antigen a strain of B. burgdorferi which expresses abundant levels of OspC. We conclude that the use of rOspC in an ELISA is a convenient, readily automated, and easily standardized test for the serodiagnosis of early Lyme disease.

MeSH Terms
Antibodies, Bacterial/blood Antigens, Bacterial Bacterial Outer Membrane Proteins/blood Borrelia burgdorferi Group/immunology Enzyme-Linked Immunosorbent Assay/methods Humans Immunoglobulin M/blood Lyme Disease/blood,diagnosis Predictive Value of Tests Serologic Tests
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Outer Membrane Proteins Immunoglobulin M OspC protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Padula S J
Department of Medicine, University of Connecticut Health Center, Farmington 06030.
Dias F
Sampieri A
Craven R B
Ryan R W
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.32.7.1733-1738.1994
Published
1994-07-00
Pages
1733-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC263779
Subset
IM
Grants
NIAMS NIH HHS · R29-AR39361 · United States
External Links
PubMed source DOI: 10.1128/jcm.32.7.1733-1738.1994 PMC Full Text View journal details
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