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PMID: 7931059 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bordetella pertussis filamentous hemagglutinin interacts with a leukocyte signal transduction complex and stimulates bacterial adherence to monocyte CR3 (CD11b/CD18).

The Journal of experimental medicine ·Vol. 180 ·No. 4 ·1994-10-01 ·Pages 1225-33

Ishibashi Y, Claus S, Relman DA

Abstract

Bordetella pertussis, the causative agent of whooping cough, adheres to human monocytes/macrophages by means of a bacterial surface-associated protein, filamentous hemagglutinin (FHA) and the leukocyte integrin, complement receptor 3 (CR3, alpha M beta 2, CD11b/CD18). We show that an FHA Arg-Gly-Asp site induces enhanced B. pertussis binding to monocytes, and that this enhancement is blocked by antibodies directed against CR3. Enhancement requires a monocyte signal transduction complex, composed of leukocyte response integrin (alpha? beta 3) and integrin-associated protein (CD47). This complex is known to upregulate CR3 binding activity. Thus, a bacterial pathogen enhances its own attachment to host cells by coopting a host cell signaling pathway.

MeSH Terms
Adhesins, Bacterial Bacterial Adhesion Base Sequence Bordetella pertussis/physiology Hemagglutinins/physiology Humans Macrophage-1 Antigen/physiology Molecular Sequence Data Monocytes/microbiology Oligopeptides/physiology Receptors, Fibronectin/physiology Signal Transduction Virulence Factors, Bordetella
Chemicals
Adhesins, Bacterial Hemagglutinins Macrophage-1 Antigen Oligopeptides Receptors, Fibronectin Virulence Factors, Bordetella filamentous hemagglutinin adhesin, Bordetella pertussis arginyl-glycyl-aspartic acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ishibashi Y
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.
Claus S
Relman D A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.180.4.1225
Published
1994-10-01
Pages
1225-33
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191706
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.180.4.1225 PMC Full Text View journal details
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