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PMID: 7931060 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

NK3-specific natural killer cells are selectively inhibited by Bw4-positive HLA alleles with isoleucine 80.

The Journal of experimental medicine ·Vol. 180 ·No. 4 ·1994-10-01 ·Pages 1235-42

Cella M, Longo A, Ferrara GB, Strominger JL, Colonna M

Abstract

Natural killer (NK) cell clones have been previously described which are inhibited by HLA-C alleles with Asn77-Lys80 (NK1-specific cells) or by HLA-C alleles with Ser77-Asn80 (NK2-specific cells). In the present work, the generation of NK cells with HLA-B-related specificities was attempted by stimulation of a Bw4 homozygous responder by a Bw6 homozygous donor. Two NK clones were found, which were inhibited by HLA-Bw4 (but not by HLA-Bw6) allotypes and by some HLA-A allotypes that share the Bw4 public epitope. Inhibition of NK cell-mediated lysis strongly correlated with the presence of an Ile residue at position 80 of the protective allele. These NK cell clones define a new specificity termed NK3.

MeSH Terms
Alleles Base Sequence Cytotoxicity, Immunologic Genes, MHC Class I HLA-B Antigens/genetics,physiology Humans Isoleucine Killer Cells, Natural/immunology Molecular Sequence Data Sensitivity and Specificity
Chemicals
HLA-B Antigens HLA-Bw4 antigen Isoleucine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cella M
Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Longo A
Ferrara G B
Strominger J L
Colonna M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.180.4.1235
Published
1994-10-01
Pages
1235-42
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191670
Subset
IM
Grants
NCI NIH HHS · CA-47554 · United States
NIDDK NIH HHS · DK-30241 · United States
External Links
PubMed source DOI: 10.1084/jem.180.4.1235 PMC Full Text View journal details
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