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PMID: 7935378 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An interaction between the DNA-binding domains of RelA(p65) and Sp1 mediates human immunodeficiency virus gene activation.

Molecular and cellular biology ·Vol. 14 ·No. 10 ·1994-10-00 ·Pages 6570-83

Perkins ND, Agranoff AB, Pascal E, Nabel GJ

Abstract

Induction of human immunodeficiency virus type 1 (HIV-1) gene expression in stimulated T cells has been attributed to the activation of the transcription factor NF-kappa B. The twice-repeated kappa B sites within the HIV-1 long terminal repeat are in close proximity to three binding sites for Sp1. We have previously shown that a cooperative interaction of NF-kappa B with Sp1 is required for the efficient stimulation of HIV-1 transcription. In this report, we define the domains of each protein responsible for this effect. Although the transactivation domains seemed likely to mediate this interaction, we find, surprisingly, that this interaction occurs through the putative DNA-binding domains of both proteins. Sp1 specifically interacted with the amino-terminal region of RelA(p65). Similarly, RelA bound directly to the zinc finger region of Sp1. This interaction was specific and resulted in cooperative DNA binding to the kappa B and Sp1 sites in the HIV-1 long terminal repeat. Furthermore, the amino-terminal region of RelA did not associate with several other transcription factors, including MyoD, E12, or Kox15, another zinc finger protein. These findings suggest that the juxtaposition of DNA-binding sites promotes a specific protein interaction between the DNA-binding regions of these transcription factors. This interaction is required for HIV transcriptional activation and may provide a mechanism to allow for selective activation of kappa B-regulated genes.

MeSH Terms
Animals Base Sequence Cells, Cultured DNA/metabolism DNA Mutational Analysis Drosophila/cytology Gene Expression Regulation, Viral HIV-1/genetics,growth & development Humans Molecular Sequence Data NF-kappa B/metabolism Protein Binding Sequence Deletion Sp1 Transcription Factor/genetics,metabolism T-Lymphocytes/microbiology Transcription Factor RelA Transcriptional Activation Zinc Fingers
Chemicals
NF-kappa B Sp1 Transcription Factor Transcription Factor RelA DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Perkins N D
Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109-0650.
Agranoff A B
Pascal E
Nabel G J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
1994-10-00
Pages
6570-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359187
Subset
IM
Grants
NIAID NIH HHS · R01 AI29179 · United States
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