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PMID: 7935454 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A critical cytoplasmic domain of the interleukin-5 (IL-5) receptor alpha chain and its function in IL-5-mediated growth signal transduction.

Molecular and cellular biology ·Vol. 14 ·No. 11 ·1994-11-00 ·Pages 7404-13

Takaki S, Kanazawa H, Shiiba M, Takatsu K

Abstract

Interleukin-5 (IL-5) regulates the production and function of B cells, eosinophils, and basophils. The IL-5 receptor (IL-5R) consists of two distinct membrane proteins, alpha and beta. The alpha chain (IL-5R alpha) is specific to IL-5. The beta chain is the common beta chain (beta c) of receptors for IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF). The cytoplasmic domains of both alpha and beta chains are essential for signal transduction. In this study, we generated cDNAs of IL-5R alpha having various mutations in their cytoplasmic domains and examined the function of these mutants by expressing them in IL-3-dependent FDC-P1 cells. The membrane-proximal proline-rich sequence of the cytoplasmic domain of IL-5R alpha, which is conserved among the alpha chains of IL-5R, IL-3R, and GM-CSF receptor (GM-CSFR), was found to be essential for the IL-5-induced proliferative response, expression of nuclear proto-oncogenes such as c-jun, c-fos, and c-myc, and tyrosine phosphorylation of cellular proteins including JAK2 protein-tyrosine kinase. In addition, analysis using chimeric receptors which consist of the extracellular domain of IL-5R alpha and the cytoplasmic domain of beta c suggested that dimerization of the cytoplasmic domain of beta c may be an important step in activating the IL-5R complex and transducing intracellular growth signals.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Division/physiology Cell Line Cytoplasm/physiology DNA Primers/genetics Gene Expression Interleukin-5/physiology Mice Molecular Sequence Data Phosphorylation Protein Conformation Proto-Oncogenes Receptors, Interleukin/chemistry,genetics,physiology Receptors, Interleukin-5 Sequence Deletion Signal Transduction/physiology
Chemicals
DNA Primers Interleukin-5 Receptors, Interleukin Receptors, Interleukin-5
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takaki S
Department of Immunology, University of Tokyo, Japan.
Kanazawa H
Shiiba M
Takatsu K
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.11.7404-7413.1994
Published
1994-11-00
Pages
7404-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359275
Subset
IM
Corrections
CommentIn
PMID: 7623858
External Links
PubMed source DOI: 10.1128/mcb.14.11.7404-7413.1994 PMC Full Text View journal details
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