Solubilization and other studies on adenylate cyclase of baker's yeast.
1. Adenylate cyclase of Saccharomyces cerevisiae was sedimented from mechanically disintegrated preparations of yeast over an unusually wide range of centrifugal forces. 2. The enzyme was readily solubilized by Ficoll and by Lubrol PX. Lubrol caused a 2-fold activation. 3. Both particle-bound and Lubrol-solubilized enzyme had an apparent Km for ATP of 1.6 mM in the presence of 0.4 mM-cyclic AMP and 5 mM-MnCl2 at pH 6.2 and 30 degrees C. 4. The Lubrol-solubilized enzyme behaved on gel filtration as a monodisperse protein with an apparent mol.wt. of about 450000.
15 references, click to expand
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Adenylate cyclase activity in lubrol-treated membranes from Neurospora crassa.Biochim Biophys Acta. 1972 Dec 7;289(2):428-32 PMID: 4265406
-
Yeast chromatin. Preparation from isolated nuclei, histone composition and transcription capacity.Eur J Biochem. 1973 Feb 15;33(1):123-30 PMID: 4570761
-
The enzymic composition of the isolated cell wall and plasma membrane of baker's yeast.Biochem J. 1970 Jan;116(1):61-9 PMID: 4312424
-
Quantitative estimation of 3'5' cyclic AMP phosphodiesterase using anion exchange resin in a batch process.Anal Biochem. 1976 Apr;71(2):623-8 PMID: 179448
-
Studies on cyclic adenosine 3' ,5'-monophosphate levels, Adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii.Arch Biochem Biophys. 1975 Aug;169(2):449-57 PMID: 170864
-
Dispersion of epinephrine sensitive and insensitive adenylate cyclase from the ciliate Tetrahymena pyriformis.Biochim Biophys Acta. 1975 Jun 24;391(2):513-6 PMID: 807249
-
Soluble and membrane-bound cyclic AMP diesterase activity with a low Michaelis constant in baker's yeast.FEBS Lett. 1975 Feb 1;50(2):283-7 PMID: 163211
-
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
-
Metabolism of adenosine 3',5'-cyclic monophosphate and induction of fruiting bodies in Coprinus macrorhizus.J Bacteriol. 1973 Mar;113(3):1249-55 PMID: 4347968
-
Demonstration in Aspergillus niger of adenyl cyclase, a cyclic adenosine 3',5'-monophosphate-binding protein, and studies on intracellular and extracellular phosphodiesterases.Can J Microbiol. 1974 Nov;20(11):1567-76 PMID: 4373154
-
Subcellular localization and some properties of the adenylate cyclase activity of the yeast, Saccharomyces cerevisiae.Biochim Biophys Acta. 1974 Nov 4;372(1):15-22 PMID: 4371861
-
A manganese-dependent adenyl cyclase in baker's yeast, Saccharomyces cerevisiae.Acta Chem Scand. 1972;26(8):3396-8 PMID: 4567259
-
Adenylate cyclase activity in Neurospora crassa. II. Kinetics.J Biol Chem. 1972 Nov 10;247(21):6880-3 PMID: 4263616
-
Adenylate cyclase activity in Neurospora crassa. I. General properties.J Biol Chem. 1972 Nov 10;247(21):6873-9 PMID: 4343160
-
Content of cyclic 3',5'-adenosine monophosphate and adenylyl cyclase in yeast at various growth conditions.Biochemistry. 1972 Jul 18;11(15):2788-91 PMID: 4339468
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