A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods.

Nucleic acids research ·Vol. 22 ·No. 18 ·1994-09-11 ·Pages 3801-5

Cobb BD, Clarkson JM

Abstract

Taguchi methods are used widely as the basis for development trials during industrial process design. Here, we describe their suitability for optimisation of the PCR. Unlike conventional strategies, these arrays revealed the effects and interactions of specific reaction components simultaneously using just a few reactions, negating the need for extensive experimental investigation. Reaction components which effected product yield were easily determined. In addition, this technique was applied to the qualitative investigation of RAPD-PCR profiles, where optimisation of the size and distribution of a number of products was determined.

Related Genes

pr1

MeSH Terms

Base Sequence Fungi/enzymology,genetics Genes, Fungal/genetics Molecular Sequence Data Polymerase Chain Reaction/methods,statistics & numerical data Research Design Serine Endopeptidases/genetics

Chemicals

Serine Endopeptidases chymoelastase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cobb B D

Microbial Pathogenicity Group, School of Biology and Biochemistry, University of Bath, UK.

Clarkson J M

References (14)

14 references, click to expand

Reverse transcription-polymerase chain reaction: an overview of the technique and its applications.
Biotechnol Adv. 1993;11(1):13-29 PMID: 14544807
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
Nucleic Acids Res. 1990 Nov 25;18(22):6531-5 PMID: 1979162
Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR.
Nucleic Acids Res. 1992 Sep 11;20(17):4567-73 PMID: 1408758
Molecular cloning and regulatory analysis of the cuticle-degrading-protease structural gene from the entomopathogenic fungus Metarhizium anisopliae.
Eur J Biochem. 1992 Mar 15;204(3):991-1001 PMID: 1551399
Direct sequencing of polymerase chain reaction-amplified DNA.
Anal Biochem. 1994 Jan;216(1):1-14 PMID: 8135340
Increased FISH efficiency using APC probes generated by direct incorporation of labeled nucleotides by PCR.
Cytogenet Cell Genet. 1994;65(3):169-71 PMID: 8222753
Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2336-40 PMID: 2006172
Fingerprinting genomes using PCR with arbitrary primers.
Nucleic Acids Res. 1990 Dec 25;18(24):7213-8 PMID: 2259619
Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA.
Curr Genet. 1991 Nov;20(5):391-6 PMID: 1807830
Genetic analysis using the polymerase chain reaction.
Annu Rev Genet. 1992;26:479-506 PMID: 1482122
Detection of molecular variation in the insect pathogenic fungus Metarhizium using RAPD-PCR.
FEMS Microbiol Lett. 1993 Sep 15;112(3):319-24 PMID: 8224797
Polymerase chain reaction-based point mutagenesis protocol.
Methods Enzymol. 1993;217:218-27 PMID: 8474332
Genome walking by single specific primer-polymerase chain reaction.
Methods Enzymol. 1993;217:436-46 PMID: 8474344
Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.
Nucleic Acids Res. 1990 Feb 25;18(4):999-1005 PMID: 2179874

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/22.18.3801

Published

1994-09-11

Pages

3801-5

Language

English

Region

England

NLM ID

0411011

PMCID

PMC308365

Subset

External Links

PubMed source DOI: 10.1093/nar/22.18.3801 PMC Full Text View journal details

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A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods.

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