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PMID: 7937129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bidirectional promoter of the mouse thymidylate synthase gene.

Nucleic acids research ·Vol. 22 ·No. 20 ·1994-10-11 ·Pages 4044-9

Liao WC, Ash J, Johnson LF

Abstract

The promoter of the mouse thymidylate synthase (TS) gene lacks both a TATAA box and an initiator element and directs transcriptional initiation at multiple sites over a 90 nucleotide initiation window. Earlier studies defined an essential region near the 5' end of the initiation window that is required for promoter activity. The essential region contains possible binding sites for Sp1 and Ets transcription factors. In the present study we show that this essential region stimulates transcription with approximately equal strength in both directions. Transcription is initiated over a broad initiation window in the reverse direction. The same elements are important for the reverse promoter and for the normal TS promoter. Sequences upstream of the essential region partially suppress expression in the reverse direction. The TS 5' flanking region, in either the normal or inverted orientation, directs S phase-specific expression of a TS minigene. This raises the possibility that an upstream gene and the TS gene may be coordinately induced at the G1/S phase boundary by a common set of control elements.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line Codon Cricetinae DNA/chemistry,metabolism Gene Expression Regulation Luciferases/genetics Mice Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger/metabolism Sp1 Transcription Factor/metabolism Thymidylate Synthase/genetics Transcription, Genetic
Chemicals
Codon RNA, Messenger Sp1 Transcription Factor DNA Luciferases Thymidylate Synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liao W C
Department of Molecular Genetics and Biochemistry, Ohio State University, Columbus 43210.
Ash J
Johnson L F
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.20.4044
Published
1994-10-11
Pages
4044-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331888
Subset
IM
Grants
NCI NIH HHS · CA16058 · United States
NIGMS NIH HHS · GM29356 · United States
External Links
PubMed source DOI: 10.1093/nar/22.20.4044 PMC Full Text View journal details
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