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PMID: 794880 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Modified recombination and transmission of mitochondrial genetic markers in rho minus mutants of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 12 ·1976-12-00 ·Pages 4608-12

Boltin-Fukuhara M, Fukuhara H

Abstract

A large number of primary petite (rho-) clones were isolated after ethidium bromide mutagenesis of various grande (rho+) strains of S. cerevisiae that contained the mitochondrial genetic markers, CR, ER, OIR (or OIIR), and PR. From the frequency of coretention of markers in the petites, we have deduced a probable circular order of the markers in the grande mitochondrial genome. From these primary clones several series of pure and stable petite clones were obtained and analyzed genetically. (a) In general, the omega allele is retained or lost together with the region carrying both CR and ER markers. (b) The petites that have retained only the CR marker fall into two classes: some have kept the omega allele of the grande strain they issued from; others exhibit a new omega expression. (c) The proportion of diploid petites in petite X grande crosses is independent of the presence of the omega allele. (d) In most cases, the coordinated transmission of markers observed so far in all grande X grande nonpolar corsses does not exist anymore in petites.

MeSH Terms
Chromosome Mapping DNA, Circular/metabolism DNA, Mitochondrial/metabolism Extrachromosomal Inheritance Mutation Recombination, Genetic Saccharomyces cerevisiae/physiology,ultrastructure
Chemicals
DNA, Circular DNA, Mitochondrial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boltin-Fukuhara M
Fukuhara H
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.12.4608
Published
1976-12-00
Pages
4608-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431565
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.12.4608 PMC Full Text View journal details
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