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PMID: 7961409 Published · ppublish English Comparative Study Journal Article

Structure and transcriptional regulation of the Escherichia coli adaptive response gene aidB.

Journal of bacteriology ·Vol. 176 ·No. 21 ·1994-11-00 ·Pages 6583-9

Landini P, Hajec LI, Volkert MR

Abstract

Expression of the Escherichia coli aidB gene is induced in vivo by alkylation damage in an ada-dependent pathway and by anaerobiosis or by acetate at pH 6.5 in an ada-independent fashion. In this report, we present data on aidB gene structure, function, and regulation. The aidB gene encodes a protein of ca. 60 kDa that is homologous to several mammalian acyl coenzyme A dehydrogenases. Accordingly, crude extracts from an aidB-overexpressing strain showed isovaleryl coenzyme A dehydrogenase activity. aidB overexpression also reduced N-methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis. Both ada- and acetate/pH-dependent induction of aidB are regulated at the transcriptional level, and the same transcriptional start point is used for both kinds of induction. Ada protein plays a direct role in aidB regulation: methylated Ada is able to bind to the aidB promoter region and to activate transcription from aidB in an in vitro transcription-translation system using crude E. coli extracts.

Related Genes
aidb
MeSH Terms
Adaptation, Physiological/genetics Alkylation Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Damage Escherichia coli/genetics Escherichia coli Proteins Fatty Acid Desaturases/genetics Gene Expression Regulation, Bacterial Isovaleryl-CoA Dehydrogenase Methylnitronitrosoguanidine Molecular Sequence Data Mutagenesis O(6)-Methylguanine-DNA Methyltransferase Oxidoreductases/metabolism Oxidoreductases Acting on CH-CH Group Donors Promoter Regions, Genetic/genetics Protein Binding Recombinant Fusion Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription Factors Transcription, Genetic
Chemicals
AidB protein, E coli Bacterial Proteins Escherichia coli Proteins Recombinant Fusion Proteins Transcription Factors Methylnitronitrosoguanidine Oxidoreductases Fatty Acid Desaturases Oxidoreductases Acting on CH-CH Group Donors Isovaleryl-CoA Dehydrogenase Ada protein, E coli O(6)-Methylguanine-DNA Methyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Landini P
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
Hajec L I
Volkert M R
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.21.6583-6589.1994
Published
1994-11-00
Pages
6583-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC197013
Subset
IM
Databases
GENBANK
L20915
External Links
PubMed source DOI: 10.1128/jb.176.21.6583-6589.1994 PMC Full Text View journal details
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