Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents.

The ada gene of Escherichia coli K-12, the regulatory locus for the adaptive response to methylating agents, coded for a 39,000-dalton protein. An adjacent gene coding for a 27,000-dalton protein was coregulated with ada. The Ada protein was strongly induced upon exposure of cells to methylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine. An analysis of ada regulation with an ada-lacZ operon fusion showed that ada+ function was required for induction of ada transcription. Derivatives of the ada gene truncated from the 3' end produced proteins which were more potent in stimulating transcription than the product of the intact ada gene, indicating that the transcription-activating function of the Ada protein resided in its amino terminus. The sequence of the ada-regulatory region and the identification of the start site of ada transcription are also presented.