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PMID: 7962063 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

NCAM-dependent neurite outgrowth is inhibited in neurons from Fyn-minus mice.

The Journal of cell biology ·Vol. 127 ·No. 3 ·1994-11-00 ·Pages 825-33

Beggs HE, Soriano P, Maness PF

Abstract

Src-related nonreceptor protein tyrosine kinases in nerve growth cones (p59fyn, pp60c-src, and pp62c-yes) are potential intracellular signaling molecules for cell adhesion molecule-directed axonal growth. To determine whether src-related tyrosine kinases mediate NCAM-dependent neurite outgrowth, cultures of cerebellar and sensory neurons from fyn-, src-, and yes- minus mice were analyzed for neurite outgrowth on monolayers of NCAM140-transfected L fibroblasts. NCAM-dependent neurite outgrowth was selectively inhibited in cultures of cerebellar and dorsal root ganglion neurons from fyn-, but not src- or yes- mice. Neurite outgrowth by fyn-, src-, or yes- neurons on untransfected fibroblast monolayers was unaffected, indicating that these kinases do not contribute significantly to axon growth on at least some integrins or other adhesive substrates present on fibroblasts. This study demonstrates that p59fyn is an essential component of the NCAM signaling pathway leading to axonal growth.

Related Genes
fyn src yes
MeSH Terms
Animals Base Sequence Cell Adhesion Molecules, Neuronal/biosynthesis,physiology Cells, Cultured Cerebellum/physiology DNA Primers Enzyme-Linked Immunosorbent Assay Kinetics L Cells Mice Mice, Inbred C57BL Mice, Neurologic Mutants Molecular Sequence Data Neurites/physiology,ultrastructure Neurons/physiology Neurons, Afferent/physiology Polymerase Chain Reaction Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/biosynthesis,genetics Proto-Oncogene Proteins c-fyn Transfection
Chemicals
Cell Adhesion Molecules, Neuronal DNA Primers Proto-Oncogene Proteins Protein-Tyrosine Kinases Fyn protein, mouse Proto-Oncogene Proteins c-fyn
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Beggs H E
Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill 27599-7260.
Soriano P
Maness P F
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.3.825
Published
1994-11-00
Pages
825-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120232
Subset
IM
Grants
NINDS NIH HHS · NS 26620 · United States
External Links
PubMed source DOI: 10.1083/jcb.127.3.825 PMC Full Text View journal details
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