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PMID: 7962075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HIV-particles in spermatozoa of patients with AIDS and their transfer into the oocyte.

The Journal of cell biology ·Vol. 127 ·No. 4 ·1994-11-00 ·Pages 903-14

Baccetti B, Benedetto A, Burrini AG, Collodel G, Ceccarini EC, Crisà N, Di Caro A, Estenoz M, Garbuglia AR, Massacesi A

Abstract

By immunocytochemistry and in situ hybridization at the electron microscopy level, and by the PCR technique, we have shown that HIV-1 binds and enters normal sperm; that viral particles, their antigens, and nucleic acid are present in sperm from HIV-1 infected men; and that such sperm can transfer HIV-1 like particles to normal human oocytes. We also present evidence that a galactosylceramide-like compound is present on the sperm membrane and could function as an alternative receptor for HIV.

MeSH Terms
Acquired Immunodeficiency Syndrome/pathology,transmission,virology Cell Membrane/physiology,ultrastructure Disease Transmission, Infectious Female Fertilization Galactosylceramides/analysis HIV-1/isolation & purification,ultrastructure Humans In Situ Hybridization Male Microscopy, Electron Oocytes/virology Polymerase Chain Reaction RNA, Viral/analysis Receptors, HIV/analysis Spermatozoa/pathology,ultrastructure,virology
Chemicals
Galactosylceramides RNA, Viral Receptors, HIV
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Baccetti B
Institute of General Biology, Consiglio Nazionale delle Ricerche, Siena, Italy.
Benedetto A
Burrini A G
Collodel G
Ceccarini E C
Crisà N
Di Caro A
Estenoz M
Garbuglia A R
Massacesi A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.4.903
Published
1994-11-00
Pages
903-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200050
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.127.4.903 PMC Full Text View journal details
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