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PMID: 7962552 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Macrophage-tropic variants initiate human immunodeficiency virus type 1 infection after sexual, parenteral, and vertical transmission.

The Journal of clinical investigation ·Vol. 94 ·No. 5 ·1994-11-00 ·Pages 2060-7

van't Wout AB, Kootstra NA, Mulder-Kampinga GA, Albrecht-van Lent N, Scherpbier HJ, Veenstra J, Boer K, Coutinho RA, Miedema F, Schuitemaker H

Abstract

Macrophage-tropic, non-syncytium-inducing, HIV-1 variants predominate in the asymptomatic phase of infection and may be responsible for establishing infection in an individual exposed to the mixture of HIV-1 variants. Here, genotypical and phenotypical characteristics of virus populations, present in sexual, parenteral, or vertical donor-recipient pairs, were studied. Sequence analysis of the V3 domain confirmed the presence of a homogeneous virus population in recently infected individuals. Biological HIV-1 clones were further characterized for syncytium inducing capacity on the MT2 cell line and for macrophage tropism as defined by the appearance of proviral DNA upon inoculation of monocyte-derived macrophages. Both sexual and parenteral transmission cases revealed a selective outgrowth in the recipient of the most macrophage-tropic variant(s) present in the donor. In three out of five vertical transmission cases, more than one highly macrophage-tropic virus variant was present in the child shortly after birth, suggestive of transmission of multiple variants. In three primary infection cases, homogeneous virus populations of macrophage-tropic, non-syncytium-inducing variants were present prior to seroconversion, thus excluding humoral immunity as the selective pressure in favour of macrophage-tropic variants. These observations may have important implications for vaccine development.

MeSH Terms
Acquired Immunodeficiency Syndrome/transmission Amino Acid Sequence Child Female HIV-1/physiology Homosexuality Humans Infectious Disease Transmission, Vertical Macrophages/virology Male Molecular Sequence Data
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
van't Wout A B
Department of Clinical Viro-Immunology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Kootstra N A
Mulder-Kampinga G A
Albrecht-van Lent N
Scherpbier H J
Veenstra J
Boer K
Coutinho R A
Miedema F
Schuitemaker H
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40 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI117560
Published
1994-11-00
Pages
2060-7
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC294642
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI117560 PMC Full Text View journal details
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