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PMID: 7962609 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

CagA/cytotoxic strains of Helicobacter pylori and interleukin-8 in gastric epithelial cell lines.

Journal of clinical pathology ·Vol. 47 ·No. 10 ·1994-10-00 ·Pages 945-50

Crabtree JE, Farmery SM, Lindley IJ, Figura N, Peichl P, Tompkins DS

Abstract

To investigate: (1) whether Helicobacter pylori directly induces interleukin-8 (IL-8) message expression and protein secretion in established gastric epithelial cell lines; and (2) if CagA/cytotoxin positive and negative strains of H pylori differ in their ability to induce epithelial IL-8. Gastric epithelial cell lines were co-cultured with H pylori NCTC 11637 and 10 clinical isolates (four cytotoxic, six non-cytotoxic) and secreted IL-8 was measured by enzyme linked immunosorbent assay (ELISA). Specific induction of gastric epithelial IL-8 mRNA was examined by reverse transcription and polymerase chain reaction (RT-PCR) amplification. H pylori (NCTC 11637) induced IL-8 secretion from three gastric epithelial cell lines (KATO-3, ST42, AGS) but not from MKN 45 (gastric) or intestinal (SW480, HT29) cell lines. H mustelae did not stimulate IL-8 secretion from KATO-3, ST42, and AGS cells. H pylori induced IL-8 secretion was reduced by heat killing, sonication, freeze thawing or formalin fixation of the bacteria. CagA/cytotoxin positive strains of H pylori induced significantly higher IL-8 secretion than CagA/cytotoxin negative strains in the three positive gastric epithelial cell lines (KATO-3, ST42: p < 0.01; AGS: p < 0.02). A significant increase (p < 0.01) in the expression of IL-8 mRNA relative to G3PDH mRNA was observed in KATO-3 cells after three hours of co-culture with CagA/cytotoxin positive strains. H pylori directly increases gastric epithelial IL-8 mRNA expression and IL-8 protein secretion in a strain specific manner. Induction of epithelial IL-8 by CagA/cytotoxin positive strains is likely to result in neutrophil chemotaxis and activation and thus mucosal damage. These observations on epithelial IL-8 may explain the association between CagA/cytotoxin positive strains and gastroduodenal disease.

MeSH Terms
Animals Antigens, Bacterial/metabolism Bacterial Proteins/metabolism Bacteriological Techniques Cell Line Epithelial Cells Epithelium/immunology,microbiology Gene Expression Regulation, Bacterial Helicobacter pylori/immunology,metabolism Interleukin-8/genetics Polymerase Chain Reaction RNA, Messenger/analysis Stomach/cytology,immunology,microbiology
Chemicals
Antigens, Bacterial Bacterial Proteins Interleukin-8 RNA, Messenger cagA protein, Helicobacter pylori
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Crabtree J E
Department of Clinical Medicine, St James's University Hospital, Leeds.
Farmery S M
Lindley I J
Figura N
Peichl P
Tompkins D S
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Article Info
Journal
Journal of clinical pathology
Abbr.
J Clin Pathol
ISSN
0021-9746
DOI
10.1136/jcp.47.10.945
Published
1994-10-00
Pages
945-50
Language
English
Region
England
NLM ID
0376601
PMCID
PMC502181
Subset
IM
External Links
PubMed source DOI: 10.1136/jcp.47.10.945 PMC Full Text View journal details
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