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PMID: 7969128 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication factor A is required in vivo for DNA replication, repair, and recombination.

Molecular and cellular biology ·Vol. 14 ·No. 12 ·1994-12-00 ·Pages 7884-90

Longhese MP, Plevani P, Lucchini G

Abstract

Replication factor A (RF-A) is a heterotrimeric single-stranded-DNA-binding protein which is conserved in all eukaryotes. Since the availability of conditional mutants is an essential step to define functions and interactions of RF-A in vivo, we have produced and characterized mutations in the RFA1 gene, encoding the p70 subunit of the complex in Saccharomyces cerevisiae. This analysis provides the first in vivo evidence that RF-A function is critical not only for DNA replication but also for efficient DNA repair and recombination. Moreover, genetic evidence indicate that p70 interacts both with the DNA polymerase alpha-primase complex and with DNA polymerase delta.

Related Genes
POL1 PRI1 RFA1
MeSH Terms
DNA Mutational Analysis DNA Polymerase II/metabolism DNA Polymerase III DNA Primase DNA Repair DNA Replication DNA, Fungal/genetics DNA-Binding Proteins/genetics DNA-Directed DNA Polymerase/metabolism Fungal Proteins/genetics RNA Nucleotidyltransferases/metabolism Recombination, Genetic Replication Protein A Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Replication Protein A DNA Primase RNA Nucleotidyltransferases DNA Polymerase II DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Longhese M P
Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.
Plevani P
Lucchini G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.12.7884-7890.1994
Published
1994-12-00
Pages
7884-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359327
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.12.7884-7890.1994 PMC Full Text View journal details
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