Home LiteratureArticle Details
PMID: 7983760 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein.

Journal of virology ·Vol. 69 ·No. 1 ·1995-01-00 ·Pages 585-8

Tong X, Wang F, Thut CJ, Kieff E

Abstract

The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic transactivator VP16 in depleting nuclear extracts of basal transcription activity. Like VP16, EBNA-2 associates with TFIIB, TAF40, and RPA70. However, EBNA-2 has much less avidity for TATA-binding protein. A Trp-to-Thr mutation within the acidic domain abolishes EBNA-2 transactivating activity and greatly compromises the association with TFIIB, TAF40, and RPA70, establishing a genetic linkage between transactivating activity and these associations.

MeSH Terms
Antigens, Viral/chemistry,metabolism Base Sequence DNA Primers DNA-Binding Proteins/chemistry,metabolism Drosophila Proteins Epstein-Barr Virus Nuclear Antigens Hydrogen-Ion Concentration Molecular Sequence Data Replication Protein A TATA Box TATA-Binding Protein Associated Factors TATA-Box Binding Protein Trans-Activators/metabolism Transcription Factor TFIIB Transcription Factor TFIID Transcription Factors/metabolism
Chemicals
Antigens, Viral DNA Primers DNA-Binding Proteins Drosophila Proteins Epstein-Barr Virus Nuclear Antigens Replication Protein A TATA-Binding Protein Associated Factors TATA-Box Binding Protein Trans-Activators Transcription Factor TFIIB Transcription Factor TFIID Transcription Factors e(y)1 protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tong X
Department of Microbiology, Harvard University, Boston, Massachusetts 02115.
Wang F
Thut C J
Kieff E
References (43)
43 references, click to expand
  1. The human homologous pairing protein HPP-1 is specifically stimulated by the cognate single-stranded binding protein hRP-A.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9067-71 PMID: 1924369
  2. A region of herpes simplex virus VP16 can substitute for a transforming domain of Epstein-Barr virus nuclear protein 2.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8030-4 PMID: 1325641
  3. The Epstein-Barr virus nuclear antigen 2 transactivator is directed to response elements by the J kappa recombination signal binding protein.
    Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7568-72 PMID: 8052621
  4. The EBNA-2 arginine-glycine domain is critical but not essential for B-lymphocyte growth transformation; the rest of region 3 lacks essential interactive domains.
    J Virol. 1994 Oct;68(10):6188-97 PMID: 8083959
  5. Wild-type p53 binds to the TATA-binding protein and represses transcription.
    Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12028-32 PMID: 1465435
  6. The Epstein-Barr virus nuclear antigen 2 interacts with an EBNA2 responsive cis-element of the terminal protein 1 gene promoter.
    EMBO J. 1993 Jan;12(1):167-75 PMID: 8381349
  7. The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication.
    Cell. 1993 Jun 18;73(6):1207-21 PMID: 8390328
  8. The transactivator proteins VP16 and GAL4 bind replication factor A.
    Cell. 1993 Jun 18;73(6):1223-32 PMID: 8513504
  9. Cell phenotype-dependent control of Epstein-Barr virus latent membrane protein 1 gene regulatory sequences.
    Virology. 1993 Jul;195(1):71-80 PMID: 8391188
  10. The Epstein-Barr virus immortalizing protein EBNA-2 is targeted to DNA by a cellular enhancer-binding protein.
    Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9237-41 PMID: 8415684
  11. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.
    Cell. 1993 Nov 5;75(3):519-30 PMID: 8221891
  12. Functional and biochemical interaction of the HTLV-I Tax1 transactivator with TBP.
    EMBO J. 1993 Nov;12(11):4269-78 PMID: 8223437
  13. Direct interaction of human TFIID with the HIV-1 transactivator tat.
    Nature. 1994 Jan 20;367(6460):295-9 PMID: 8121496
  14. Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein J kappa.
    Science. 1994 Jul 1;265(5168):92-5 PMID: 8016657
  15. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  16. Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4394-8 PMID: 3925456
  17. Replication of simian virus 40 origin-containing DNA in vitro with purified proteins.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1834-8 PMID: 3031654
  18. Epstein-Barr virus nuclear antigen 2 specifically induces expression of the B-cell activation antigen CD23.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3452-6 PMID: 3033649
  19. Cellular factors required for multiple stages of SV40 DNA replication in vitro.
    EMBO J. 1988 Apr;7(4):1211-8 PMID: 2841119
  20. Purification and characterization of a protein from Saccharomyces cerevisiae that binds tightly to single-stranded DNA and stimulates a cognate strand exchange protein.
    Biochemistry. 1989 Apr 4;28(7):2856-62 PMID: 2663063
  21. Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes.
    Nature. 1989 Aug 3;340(6232):393-7 PMID: 2547164
  22. Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9558-62 PMID: 2556717
  23. Stable transfection of Epstein-Barr virus (EBV) nuclear antigen 2 in lymphoma cells containing the EBV P3HR1 genome induces expression of B-cell activation molecules CD21 and CD23.
    J Virol. 1990 Mar;64(3):1002-13 PMID: 2154588
  24. Epstein-Barr virus nuclear antigen 2 induces expression of the virus-encoded latent membrane protein.
    J Virol. 1990 May;64(5):2126-34 PMID: 2157875
  25. Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are effectors of phenotypic changes in B lymphocytes: EBNA-2 and LMP1 cooperatively induce CD23.
    J Virol. 1990 May;64(5):2309-18 PMID: 2157887
  26. The level of c-fgr RNA is increased by EBNA-2, an Epstein-Barr virus gene required for B-cell immortalization.
    J Virol. 1990 Jun;64(6):2530-6 PMID: 2159528
  27. Epstein-Barr virus nuclear antigen 2 transactivates latent membrane protein LMP1.
    J Virol. 1990 Jul;64(7):3407-16 PMID: 2352328
  28. An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A.
    EMBO J. 1990 Jul;9(7):2321-9 PMID: 2192864
  29. Epstein-Barr virus nuclear antigen 2 activates transcription of the terminal protein gene.
    J Virol. 1991 Jan;65(1):415-23 PMID: 1845900
  30. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.
    Cell. 1991 Feb 8;64(3):521-32 PMID: 1825028
  31. Requirement for the replication protein SSB in human DNA excision repair.
    Nature. 1991 Feb 7;349(6309):538-41 PMID: 1992355
  32. EBNA-2 transactivates a lymphoid-specific enhancer in the BamHI C promoter of Epstein-Barr virus.
    J Virol. 1991 May;65(5):2164-9 PMID: 1850003
  33. Epstein-Barr virus nuclear protein 2 mutations define essential domains for transformation and transactivation.
    J Virol. 1991 May;65(5):2545-54 PMID: 1850028
  34. Reduced binding of TFIID to transcriptionally compromised mutants of VP16.
    Nature. 1991 Jun 13;351(6327):588-90 PMID: 1646402
  35. Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication.
    J Biol Chem. 1991 Jun 25;266(18):12090-8 PMID: 2050703
  36. Epstein-Barr virus nuclear protein 2 transactivates a cis-acting CD23 DNA element.
    J Virol. 1991 Aug;65(8):4101-6 PMID: 1649318
  37. Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor.
    Cell. 1991 Oct 18;67(2):365-76 PMID: 1833071
  38. An Epstein-Barr virus nuclear protein 2 domain essential for transformation is a direct transcriptional activator.
    J Virol. 1991 Nov;65(11):5880-5 PMID: 1656076
  39. Binding of general transcription factor TFIIB to an acidic activating region.
    Nature. 1991 Oct 10;353(6344):569-71 PMID: 1922364
  40. Binding properties of replication protein A from human and yeast cells.
    Mol Cell Biol. 1992 Jul;12(7):3050-9 PMID: 1320195
  41. In vitro transcription faithfully reflecting T-cell activation requirements.
    J Biol Chem. 1992 Jan 5;267(1):399-406 PMID: 1730605
  42. Genetic and biochemical evidence that EBNA 2 interaction with a 63-kDa cellular GTG-binding protein is essential for B lymphocyte growth transformation by EBV.
    Virology. 1994 Nov 1;204(2):634-41 PMID: 7941331
  43. Delineation of the cis-acting element mediating EBNA-2 transactivation of latent infection membrane protein expression.
    J Virol. 1991 Dec;65(12):6765-71 PMID: 1658373
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.1.585-588.1995
Published
1995-01-00
Pages
585-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC188615
Subset
IM
Grants
NCI NIH HHS · CA 47006 · United States
NCI NIH HHS · CA 52244 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.1.585-588.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2