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PMID: 7988560 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human J kappa recombination signal sequence binding protein (RBP-J kappa) targets the Epstein-Barr virus EBNA2 protein to its DNA responsive elements.

The EMBO journal ·Vol. 13 ·No. 23 ·1994-12-01 ·Pages 5633-8

Waltzer L, Logeat F, Brou C, Israel A, Sergeant A, Manet E

Abstract

The Epstein-Barr virus (EBV) protein EBNA2, which is essential for the immortalization of human primary B cells by EBV, acts as a transcriptional activator of cellular and viral genes. Specific responsive elements have been characterized in several of the promoters activated by EBNA2. They all share the core sequence GTGGGAA. EBNA2 does not, however, bind to these sequences directly, but appears to be targeted to them by a cellular protein. A similar core sequence has recently been identified as a high-affinity binding site for the human recombination signal sequence binding protein RBP-J kappa. Here we provide evidence that RBP-J kappa binds to specific sequences in EBNA2-responsive elements. Our results also demonstrate that RBP-J kappa makes direct physical contact with EBNA2 in solution and recruits EBNA2 to its cognate DNA sequences, suggesting that RBP-J kappa may mediate EBNA2 transactivation of both cellular and viral genes.

MeSH Terms
Antigens, Viral/metabolism Base Sequence DNA/metabolism DNA, Viral/metabolism DNA-Binding Proteins/metabolism Epstein-Barr Virus Nuclear Antigens HeLa Cells Herpesvirus 4, Human/genetics,metabolism Humans Immunoglobulin J Recombination Signal Sequence-Binding Protein Molecular Sequence Data Nuclear Proteins Protein Binding Regulatory Sequences, Nucleic Acid Transcriptional Activation
Chemicals
Antigens, Viral DNA, Viral DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens Immunoglobulin J Recombination Signal Sequence-Binding Protein Nuclear Proteins RBPJ protein, human DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Waltzer L
Unité de Virologie Humaine, ENS-INSERM U412, Ecole Normale Supérieure de Lyon, France.
Logeat F
Brou C
Israel A
Sergeant A
Manet E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-12-01
Pages
5633-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395528
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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