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PMID: 8013470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Copy-choice illegitimate DNA recombination revisited.

The EMBO journal ·Vol. 13 ·No. 11 ·1994-06-01 ·Pages 2725-34

d'Alençon E, Petranovic M, Michel B, Noirot P, Aucouturier A, Uzest M, Ehrlich SD

Abstract

Nearly precise excision of a transposon related to Tn10 from an Escherichia coli plasmid was used as a model to study illegitimate DNA recombination between short direct repeats. The excision was stimulated 100-1000 times by induction of plasmid single-stranded DNA synthesis and did not involve transfer of DNA from the parental to the progeny molecule. We conclude that it occurred by copy-choice DNA recombination, and propose that other events of recombination between short direct repeats might be a result of the same process.

MeSH Terms
Base Sequence DNA Replication/genetics DNA Transposable Elements/genetics DNA, Recombinant/metabolism DNA, Single-Stranded/biosynthesis Escherichia coli/genetics,growth & development Inoviridae/physiology Models, Genetic Molecular Sequence Data Multigene Family/genetics Nucleic Acid Conformation Plasmids/genetics,metabolism Recombination, Genetic/genetics Repetitive Sequences, Nucleic Acid Viral Proteins/genetics,metabolism
Chemicals
DNA Transposable Elements DNA, Recombinant DNA, Single-Stranded Viral Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
d'Alençon E
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Petranovic M
Michel B
Noirot P
Aucouturier A
Uzest M
Ehrlich S D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-06-01
Pages
2725-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395147
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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