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PMID: 8019000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cdc25 is one of the MPM-2 antigens involved in the activation of maturation-promoting factor.

Molecular biology of the cell ·Vol. 5 ·No. 2 ·1994-02-00 ·Pages 135-45

Kuang J, Ashorn CL, Gonzalez-Kuyvenhoven M, Penkala JE

Abstract

MPM-2 antigens, a discrete set of phosphoproteins that contain similar phosphoepitopes recognized by the monoclonal antibody MPM-2, are phosphorylated during M-phase induction. Our previous studies suggested that certain MPM-2 antigens are involved in the appearance of maturation-promoting factor (MPF) activity. Because the central mitotic regulator cdc2 kinase has been shown to exhibit MPF activity, we explored the possibility that certain MPM-2 antigens are regulators of cdc2 kinase. We found that MPM-2 binding of its antigens would inhibit the autoamplification of cdc2 kinase in Xenopus oocytes and interfere with cyclin-activation of cdc2 kinase in Xenopus interphase egg extract. Immunodepletion of MPM-2 antigens from cyclin-induced M-phase egg extract caused the inactivation of cdc2 kinase, which was accompanied by an inhibitory phosphorylation of p34cdc2 on Thr 14 and Tyr 15, indicating that at least one MPM-2 antigen is a positive regulator of p34cdc2 dephosphorylation. We then showed that cdc25 from M-phase arrested egg extract is an MPM-2 antigen. These results suggest that phosphorylation of the epitope recognized by MPM-2 may be a crucial event in the activation of cdc25 and that the kinase(s) that phosphorylates this MPM-2 epitope may be an important regulator of cdc2 kinase activation.

MeSH Terms
Animals Antigens/metabolism CDC2 Protein Kinase/antagonists & inhibitors,metabolism Cyclins/metabolism Enzyme Activation Maturation-Promoting Factor/metabolism Mitosis Oocytes/enzymology Phosphoprotein Phosphatases/metabolism Phosphorylation Xenopus cdc25 Phosphatases
Chemicals
Antigens Cyclins CDC2 Protein Kinase Maturation-Promoting Factor Phosphoprotein Phosphatases cdc25 Phosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuang J
Department of Clinical Investigation, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Ashorn C L
Gonzalez-Kuyvenhoven M
Penkala J E
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.5.2.135
Published
1994-02-00
Pages
135-45
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301020
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1091/mbc.5.2.135 PMC Full Text View journal details
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