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PMID: 3821728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Metaphase protein phosphorylation in Xenopus laevis eggs.

Molecular and cellular biology ·Vol. 7 ·No. 2 ·1987-02-00 ·Pages 760-8

Lohka MJ, Kyes JL, Maller JL

Abstract

Cytoplasmic extracts of metaphase (M-phase)-arrested Xenopus laevis eggs support nuclear envelope breakdown and chromosome condensation in vitro. Induction of nuclear breakdown is inhibited by AMPP(NH)P, a nonhydrolyzable ATP analog, but not by ATP or gamma-S-ATP, a hydrolyzable ATP analog, suggesting that protein phosphorylation may be required for M-phase nuclear events in vitro. By addition of [gamma-32P]ATP, we have identified in cytoplasmic extracts and in intact eggs at least six phosphoproteins that are present during M-phase but absent in G1/S-phase. These phosphoproteins also appear in response to partially purified preparations of maturation-promoting factor. A subset of these proteins are thiophosphorylated by gamma-S-ATP under conditions that promote nuclear envelope breakdown and chromosome condensation. Each of these proteins is phosphorylated on serine and threonine, and one, a 42-kilodalton protein, is also phosphorylated on tyrosine both in extracts and in intact eggs. These results indicate that activation of protein kinases accounts for at least part of the increased phosphorylation in M-phase and that both protein-serine-threonine kinases and protein-tyrosine kinases may play a role in controlling M-phase nuclear behavior.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Cell Nucleus/physiology,ultrastructure Chromosomes/ultrastructure Cytoplasm/physiology Interphase Isoelectric Point Metaphase Molecular Weight Nuclear Envelope/physiology Phosphoproteins/physiology Phosphorylation Xenopus laevis
Chemicals
Phosphoproteins Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lohka M J
Kyes J L
Maller J L
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.2.760-768.1987
Published
1987-02-00
Pages
760-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365132
Subset
IM
Grants
NIGMS NIH HHS · GM26743 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.2.760-768.1987 PMC Full Text View journal details
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