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PMID: 8022787 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA-binding and trans-activation properties of Drosophila E2F and DP proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 14 ·1994-07-05 ·Pages 6359-63

Dynlacht BD, Brook A, Dembski M, Yenush L, Dyson N

Abstract

The temporal activation of E2F transcriptional activity appears to be an important component of the mechanisms that prepare mammalian cells for DNA replication. Regulation of E2F activity appears to be a highly complex process, and the dissection of the E2F pathway will be greatly facilitated by the ability to use genetic approaches. We report the isolation of two Drosophila genes that can stimulate E2F-dependent transcription in Drosophila cells. One of these genes, dE2F, contains three domains that are highly conserved in the human homologs E2F-1, E2F-2, and E2F-3. Interestingly, one of these domains is highly homologous to the retinoblastoma protein (RB)-binding sequences of human E2F genes. The other gene, dDP, is closely related to the human DP-1 and DP-2 genes. We demonstrate that dDP and dE2F interact and cooperate to give sequence-specific DNA binding and optimal trans-activation. These features suggest that endogenous Drosophila E2F, like human E2F, may be composed of heterodimers and may be regulated by RB-like proteins. The isolation of these genes will provide important reagents for the genetic analysis of the E2F pathway.

MeSH Terms
Amino Acid Sequence Animals Carrier Proteins Cell Cycle Proteins Cell Line Cloning, Molecular Conserved Sequence DNA, Complementary/metabolism DNA-Binding Proteins/biosynthesis,metabolism Drosophila/genetics,metabolism Drosophila Proteins E2F Transcription Factors E2F1 Transcription Factor E2F2 Transcription Factor E2F3 Transcription Factor Humans Mice Molecular Sequence Data Plasmids Retinoblastoma-Binding Protein 1 Sequence Homology, Amino Acid Trans-Activators/biosynthesis,metabolism Transcription Factor DP1 Transcription Factors/biosynthesis,metabolism Transfection
Chemicals
Arid4a protein, mouse Carrier Proteins Cell Cycle Proteins DNA, Complementary DNA-Binding Proteins Dp transcription factor, Drosophila Drosophila Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human E2F2 Transcription Factor E2F2 protein, human E2F3 Transcription Factor E2f1 protein, mouse Retinoblastoma-Binding Protein 1 TFDP1 protein, human Trans-Activators Transcription Factor DP1 Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dynlacht B D
Laboratory of Molecular Oncology, Massachusetts General Hospital Cancer Center, Charlestown 02129.
Brook A
Dembski M
Yenush L
Dyson N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.14.6359
Published
1994-07-05
Pages
6359-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44201
Subset
IM
Databases
GENBANK
X78421, X79708
External Links
PubMed source DOI: 10.1073/pnas.91.14.6359 PMC Full Text View journal details
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