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PMID: 8246996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The retinoblastoma protein binds to a family of E2F transcription factors.

Molecular and cellular biology ·Vol. 13 ·No. 12 ·1993-12-00 ·Pages 7813-25

Lees JA, Saito M, Vidal M, Valentine M, Look T, Harlow E, Dyson N, Helin K

Abstract

E2F is a transcription factor that helps regulate the expression of a number of genes that are important in cell proliferation. Recently, several laboratories have isolated a cDNA clone that encodes an E2F-like protein, known as E2F-1. Subsequent characterization of this protein showed that it had the properties of E2F, but it was difficult to account for all of the suggested E2F activities through the function of this one protein. Using low-stringency hybridization, we have isolated cDNA clones that encode two additional E2F-like proteins, called E2F-2 and E2F-3. The chromosomal locations of the genes for E2F-2 and E2F-3 were mapped to 1p36 and 6q22, respectfully, confirming their independence from E2F-1. However, the E2F-2 and E2F-3 proteins are closely related to E2F-1. Both E2F-2 and E2F-3 bound to wild-type but not mutant E2F recognition sites, and they bound specifically to the retinoblastoma protein in vivo. Finally, E2F-2 and E2F-3 were able to activate transcription of E2F-responsive genes in a manner that was dependent upon the presence of at least one functional E2F binding site. These observations suggest that the E2F activities described previously result from the combined action of a family of proteins.

Related Genes
E2F E2F-1 E2F-2 E2F-3
MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites/genetics Carrier Proteins Cell Cycle Proteins Cell Line Chromosome Mapping Cloning, Molecular DNA/metabolism DNA Primers/genetics DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F2 Transcription Factor E2F3 Transcription Factor Gene Expression Regulation Humans In Vitro Techniques Molecular Sequence Data Protein Binding Retinoblastoma Protein/metabolism Retinoblastoma-Binding Protein 1 Sequence Homology, Amino Acid Transcription Factor DP1 Transcription Factors/genetics,metabolism Transcriptional Activation
Chemicals
Carrier Proteins Cell Cycle Proteins DNA Primers DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human E2F2 Transcription Factor E2F2 protein, human E2F3 Transcription Factor Retinoblastoma Protein Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lees J A
Massachusetts General Hospital Cancer Center, Charlestown 02129.
Saito M
Vidal M
Valentine M
Look T
Harlow E
Dyson N
Helin K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.12.7813-7825.1993
Published
1993-12-00
Pages
7813-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364853
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.12.7813-7825.1993 PMC Full Text View journal details
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