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PMID: 8246995 Published · ppublish English Journal Article

Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F.

Molecular and cellular biology ·Vol. 13 ·No. 12 ·1993-12-00 ·Pages 7802-12

Ivey-Hoyle M, Conroy R, Huber HE, Goodhart PJ, Oliff A, Heimbrook DC

Abstract

E2F is a mammalian transcription factor that appears to play an important role in cell cycle regulation. While at least two proteins (E2F-1 and DP-1) with E2F-like activity have been cloned, studies from several laboratories suggest that additional homologs may exist. A novel protein with E2F-like properties, designated E2F-2, was cloned by screening a HeLa cDNA library with a DNA probe derived from the DNA binding domain of E2F-1 (K. Helin, J. A. Lees, M. Vidal, N. Dyson, E. Harlow, and A. Fattaey, Cell 70:337-350, 1992). E2F-2 exhibits overall 46% amino acid identity to E2F-1. Both the sequence and the function of the DNA and retinoblastoma gene product binding domains of E2F-1 are conserved in E2F-2. The DNA binding activity of E2F-2 is dramatically enhanced by complementation with particular sodium dodecyl sulfate-polyacrylamide gel electrophoresis-purified components of HeLa cell E2F, and anti-E2F-2 antibodies cross-react with components of purified HeLa cell E2F. These observations are consistent with a model in which E2F binds DNA as a heterodimer of two distinct proteins, and E2F-2 is functionally and immunologically related to one of these proteins.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites/genetics Carrier Proteins Cell Cycle Proteins Cloning, Molecular Conserved Sequence DNA Primers/genetics DNA, Complementary/genetics DNA-Binding Proteins/chemistry,genetics,metabolism E2F Transcription Factors E2F1 Transcription Factor E2F2 Transcription Factor Glutathione Transferase/genetics HeLa Cells Humans Molecular Sequence Data Protein Conformation RNA, Messenger/genetics Recombinant Fusion Proteins/genetics Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors/chemistry,genetics,metabolism
Chemicals
Carrier Proteins Cell Cycle Proteins DNA Primers DNA, Complementary DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human E2F2 Transcription Factor E2F2 protein, human RNA, Messenger Recombinant Fusion Proteins Retinoblastoma-Binding Protein 1 TFDP1 protein, human Transcription Factor DP1 Transcription Factors Glutathione Transferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ivey-Hoyle M
Department of Cancer Research, Merck Research Laboratories, West Point, Pennsylvania 19486.
Conroy R
Huber H E
Goodhart P J
Oliff A
Heimbrook D C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.12.7802-7812.1993
Published
1993-12-00
Pages
7802-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364852
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.12.7802-7812.1993 PMC Full Text View journal details
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