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PMID: 8230483 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1.

Journal of virology ·Vol. 67 ·No. 12 ·1993-12-00 ·Pages 7641-7

Dyson N, Dembski M, Fattaey A, Ngwu C, Ewen M, Helin K

Abstract

The binding of viral oncogenes to cellular proteins is thought to modulate the activities of these cellular targets. The p107 protein is targeted by many viral proteins, including adenovirus E1A, simian virus 40 large T antigen, and human papillomavirus type 16 E7 protein. A panel of monoclonal antibodies against p107 was raised and used to identify cellular proteins that interact with the p107 protein in vivo. p107-associated proteins included cyclin A, cyclin E, and cdk2. In addition, p107 was found to associate with 62- to 65- and 50-kDa phosphoproteins in ML-1 cells, a human myeloid leukemia cell line. The 62- to 65-kDa proteins have many of the properties of the transcription factor E2F but were distinguished from pRB-associated E2F-1 by both immunologic and biochemical properties.

MeSH Terms
Antibodies, Monoclonal Carrier Proteins/immunology Cell Cycle Proteins Cross Reactions DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor Humans Nuclear Proteins Proteins/immunology Retinoblastoma-Binding Protein 1 Retinoblastoma-Like Protein p107 Transcription Factor DP1 Transcription Factors/immunology Tumor Cells, Cultured
Chemicals
Antibodies, Monoclonal Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human Nuclear Proteins Proteins RBL1 protein, human Retinoblastoma-Binding Protein 1 Retinoblastoma-Like Protein p107 Transcription Factor DP1 Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dyson N
Massachusetts General Hospital Cancer Center, Charlestown 02129.
Dembski M
Fattaey A
Ngwu C
Ewen M
Helin K
References (51)
51 references, click to expand
  1. Monoclonal antibodies specific for simian virus 40 tumor antigens.
    J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
  2. A cellular protein that competes with SV40 T antigen for binding to the retinoblastoma gene product.
    Nature. 1991 Mar 14;350(6314):160-2 PMID: 2005966
  3. Detection of cellular proteins associated with human adenovirus type 5 early region 1A polypeptides.
    Virology. 1985 Nov;147(1):142-53 PMID: 2932846
  4. Association of adenovirus early-region 1A proteins with cellular polypeptides.
    Mol Cell Biol. 1986 May;6(5):1579-89 PMID: 2431282
  5. Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
    Cell. 1988 Jul 1;54(1):17-26 PMID: 3289755
  6. Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product.
    Nature. 1988 Jul 14;334(6178):124-9 PMID: 2968522
  7. Human cyclin A and the retinoblastoma protein interact with similar but distinguishable sequences in the adenovirus E1A gene product.
    Oncogene. 1991 Mar;6(3):481-5 PMID: 1826347
  8. The E2F transcription factor is a cellular target for the RB protein.
    Cell. 1991 Jun 14;65(6):1053-61 PMID: 1828392
  9. The retinoblastoma protein copurifies with E2F-I, an E1A-regulated inhibitor of the transcription factor E2F.
    Cell. 1991 Jun 14;65(6):1063-72 PMID: 1828393
  10. Adenovirus E1a prevents the retinoblastoma gene product from complexing with a cellular transcription factor.
    Nature. 1991 Jun 6;351(6326):494-7 PMID: 1710781
  11. Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A.
    Cell. 1991 Jun 28;65(7):1243-53 PMID: 1829647
  12. Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product.
    Nature. 1991 Jul 18;352(6332):251-4 PMID: 1857421
  13. Amino-terminal domains of c-myc and N-myc proteins mediate binding to the retinoblastoma gene product.
    Nature. 1991 Aug 8;352(6335):541-4 PMID: 1865909
  14. Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.
    Nature. 1991 Sep 12;353(6340):174-7 PMID: 1653904
  15. Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A.
    Science. 1991 Sep 13;253(5025):1271-5 PMID: 1653969
  16. Molecular cloning, chromosomal mapping, and expression of the cDNA for p107, a retinoblastoma gene product-related protein.
    Cell. 1991 Sep 20;66(6):1155-64 PMID: 1833063
  17. Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
    Mol Cell Biol. 1991 Nov;11(11):5792-9 PMID: 1717832
  18. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F.
    Nature. 1992 Jan 9;355(6356):176-9 PMID: 1530885
  19. The transcription factor E2F interacts with the retinoblastoma product and a p107-cyclin A complex in a cell cycle-regulated manner.
    Cell. 1992 Jan 10;68(1):157-66 PMID: 1531040
  20. A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex.
    Cell. 1992 Jan 10;68(1):167-76 PMID: 1310073
  21. The retinoblastoma protein physically associates with the human cdc2 kinase.
    Mol Cell Biol. 1992 Mar;12(3):971-80 PMID: 1545827
  22. Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins.
    Science. 1992 Jan 3;255(5040):85-7 PMID: 1532457
  23. Interaction between human cyclin A and adenovirus E1A-associated p107 protein.
    Science. 1992 Jan 3;255(5040):87-90 PMID: 1532458
  24. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.
    Cell. 1992 Jul 24;70(2):337-50 PMID: 1638634
  25. Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties.
    Cell. 1992 Jul 24;70(2):351-64 PMID: 1638635
  26. Retinoblastoma gene product activates expression of the human TGF-beta 2 gene through transcription factor ATF-2.
    Nature. 1992 Jul 23;358(6384):331-4 PMID: 1641004
  27. Phosphorylation of the retinoblastoma protein by cdk2.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7900-4 PMID: 1518810
  28. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.
    Genes Dev. 1992 Oct;6(10):1874-85 PMID: 1398067
  29. Identification of a 60-kilodalton Rb-binding protein, RBP60, that allows the Rb-E2F complex to bind DNA.
    Mol Cell Biol. 1992 Oct;12(10):4327-33 PMID: 1406625
  30. E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.
    Science. 1992 Oct 16;258(5081):424-9 PMID: 1411535
  31. Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: identification of a gene with properties of the transcription factor E2F.
    Mol Cell Biol. 1992 Dec;12(12):5620-31 PMID: 1448092
  32. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  33. Interaction of myogenic factors and the retinoblastoma protein mediates muscle cell commitment and differentiation.
    Cell. 1993 Feb 12;72(3):309-24 PMID: 8381715
  34. The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB.
    Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1580-4 PMID: 8434021
  35. A new component of the transcription factor DRTF1/E2F.
    Nature. 1993 Mar 4;362(6415):83-7 PMID: 8446173
  36. Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4.
    Genes Dev. 1993 Mar;7(3):331-42 PMID: 8449399
  37. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.
    Genes Dev. 1993 Apr;7(4):555-69 PMID: 8384581
  38. The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2.
    J Virol. 1993 May;67(5):2456-65 PMID: 8386263
  39. Transcription factor E2F binds DNA as a heterodimer.
    Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3525-9 PMID: 8475102
  40. Functional interactions of the retinoblastoma protein with mammalian D-type cyclins.
    Cell. 1993 May 7;73(3):487-97 PMID: 8343202
  41. Physical interaction of the retinoblastoma protein with human D cyclins.
    Cell. 1993 May 7;73(3):499-511 PMID: 8490963
  42. GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.
    Science. 1993 May 21;260(5111):1130-2 PMID: 8493557
  43. The retinoblastoma protein binds to a family of E2F transcription factors.
    Mol Cell Biol. 1993 Dec;13(12):7813-25 PMID: 8246996
  44. SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene.
    Cell. 1988 Jul 15;54(2):275-83 PMID: 2839300
  45. Cellular targets for transformation by the adenovirus E1A proteins.
    Cell. 1989 Jan 13;56(1):67-75 PMID: 2521301
  46. Mapping of cellular protein-binding sites on the products of early-region 1A of human adenovirus type 5.
    Mol Cell Biol. 1988 Sep;8(9):3955-9 PMID: 2975755
  47. The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product.
    Science. 1989 Feb 17;243(4893):934-7 PMID: 2537532
  48. An N-terminal transformation-governing sequence of SV40 large T antigen contributes to the binding of both p110Rb and a second cellular protein, p120.
    Cell. 1989 Jul 28;58(2):257-67 PMID: 2526683
  49. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product.
    EMBO J. 1989 Dec 20;8(13):4099-105 PMID: 2556261
  50. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.
    Cell. 1991 Feb 8;64(3):521-32 PMID: 1825028
  51. Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.
    J Virol. 1985 Sep;55(3):533-46 PMID: 3894685
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.12.7641-7647.1993
Published
1993-12-00
Pages
7641-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238233
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.67.12.7641-7647.1993 PMC Full Text View journal details
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